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Table 2 Primers used in this study

From: GeXP analyzer-based multiplex reverse-transcription PCR assay for the simultaneous detection and differentiation of eleven duck viruses

Virus

Forward primer sequence (5′−3′)

Reverse primer sequence (5′−3′)

Amplicon size (bp)

Target region

AIV-M

AGGTGACACTATAGAATA CAGAAACGGATGGGAGTGC

GTACGACTCACTATAGGGA TATCAAGTGCAAGATCCCAATGAT

122

M

AIV-H5

AGGTGACACTATAGAATA CTTCAGGCATCAAAATGCACA

GTACGACTCACTATAGGGA TAGTTTGTTCATTTCTGAGTCGGTC

285

HA

AIV-H7

AGGTGACACTATAGAATA AATGGGGCHTTCATAGCTCC

GTACGACTCACTATAGGGA TGATAGCARTCRCCTTCACAA

144

HA

AIV-H9

AGGTGACACTATAGAATA ACAACAAGTGTGACAACAGAAGA

GTACGACTCACTATAGGGA TCTTCCGTGGCTCTCTCC

237

HA

DHV

AGGTGACACTATAGAATA TCTTCGTTGTGAAACGGATTACC

GTACGACTCACTATAGGGA TGCCTGGACAGATDTGTGCCTACT

133

5′ UTR

DTMUV

AGGTGACACTATAGAATA ATGGACAGGGTCATCAGCGG

GTACGACTCACTATAGGGA GAATRGCTCCYGCCAATGCT

176

E

EDSV

AGGTGACACTATAGAATA AATCGGCAACTCAAGACATC

GTACGACTCACTATAGGGA CCCATTCATAAACAGGATTC

208

Penton

DEV

AGGTGACACTATAGAATA GGGAGGAGCAAACAAAGA

GTACGACTCACTATAGGGA ATCGCAAATTCCATCACATA

150

UL6

NDV

AGGTGACACTATAGAATA GTRGCAGCAAGRACAAGG

GTACGACTCACTATAGGGA CATATCYGCATACATCAA

196

L

DuCV

AGGTGACACTATAGAATA TGCKCCAAAGAGTCGACATA

GTACGACTCACTATAGGGA CAAAYGCATAACGGCTCTTTCC

300

Red

MDRV

AGGTGACACTATAGAATA CAGTTGAGCCGGAYGGTAATT

GTACGACTCACTATAGGGA ACTCGGTTGGTGTTAGTVGCVTAGAA

219

S1

MDPV

AGGTGACACTATAGAATA CTTTCAGGCTACATCTTCAA

GTACGACTCACTATAGGGA AATTCTCTTTTCACCCATCC

253

VP1

Universal tag sequences Tag-F: AGGTGACACTATAGAATA Tag-R: GTACGACTCACTATAGGGA

  
  1. Universal tag sequences are underlined. Bold type indicates degenerate sites
  2. Abbreviations: M A or C, R A or G, W A or T, S G or C, Y C or T, K G or T, V A, G, or C, H A, C, or T, D A, G, or T; B G, C, or T
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BMC Microbiology

ISSN: 1471-2180

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