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Table 3 Determination of free thiol groups in protein fusions

From: Characterization of two Lactococcus lactis zinc membrane proteins, Llmg_0524 and Llmg_0526, and role of Llmg_0524 in cell wall integrity

  MalE-524Nter MalE-524Nter, zinc MalE-526Nter MalE-526Nter, zinc
Thiol (μM) 17 ± 3 17 ± 2 8 ± 1 5 ± 0.5
Thiol/protein 0.85 ± 0.15 0.85 ± 0.1 0.4 ± 0.05 0.25 ± 0.02
  1. 20 μM of protein (equivalent to 80 μM of free thiol groups) was used for thiol group determination by DTNB assays