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Table 2 Llmg_0524 and Llmg_0526 are zinc metalloproteins

From: Characterization of two Lactococcus lactis zinc membrane proteins, Llmg_0524 and Llmg_0526, and role of Llmg_0524 in cell wall integrity

Protein Ratio [Metal]/[protein] Ratio [Zinc(PAR)2]/[protein]
  Mg Fe Zn  
MalE-0524Nter 0.21 ± 0.05 0.07 ± 0.01 0.45 ± 0.02 0.5 ± 0.07
MalE-0526 Nter 0.26 ± 0.02 0.54 ± 0.05 0.79 ± 0.05 0.8 ± 0.10
MalE-lacZα 0.58 ± 0.1 0 0.04 ± 0.01 ND
+ zinc     
MalE-0524 Nter 0.20 ± 0.07 0.02 ± 0.01 0.42 ± 0.08 0.4 ± 0.03
MalE-0526 Nter 0.30 ± 0.11 0.03 ± 0.01 0.65 ± 0.14 0.8 ± 0.05
MalE-lacZα 0.17 ± 0.04 0 0.01 ± 0.01 ND
  1. All Buffers were treated with Chelex. Values are the mean of three independent protein purifications. + zinc, cultures were supplemented with 0.1 mM of zinc salt. MalE-lacZα (from plasmid, pMal c4x) is used as a control, ND: not determined