Fig. 6
Role of Llmg_0524 in the cell wall integrity. a Effect on the growth of the deletion of the cysteine-rich motif in Llmg_0524. Cells were grown in M17glucose in static conditions at 30 °C. The ∆llmg_0524 mutant carrying the plasmid pllmg_0524 c,∆cyst is represented by a dark gray dashed line. The plasmid encoded the full operon, but lacked the region in the llmg_0524 ORF corresponding to the cysteine motif. Values, plus standard deviations, are the means of three independent experiments (same results were obtained with galactose as an energy source). b Effect of lysozyme resistance of the deletion of the cysteine-rich motif in Llmg_0524. After overnight cultures, strains were washed in PBS buffer and recovered at the same OD600. After 60 min of incubation in absence (white bars) or in presence of lysozyme (black bars), SDS was added at 0.1 % final concentration and measured OD600. Values, plus standard deviation, are the means of three independent experiments. They are expressed in percent of OD600 measured in samples incubated 60 min in absence of lysozyme and SDS