Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species

BMC Microbiology

Table 1 Cabohydrate composition analysis of LPS of pathogenic versus intermediate pathogenic Leptospira strains

Sugar	Pathogenic L1-130			Intermediate pathogenic VAR010
Sugar	AQ LPS	PH LPS	Ave (SD)	AQ LPS	PH LPS	Ave (SD)
Arabinose (Ara)	20.45	20.56	20.5 (0.05)	39.32	33.52	36.4 (5.8)
Rhamnose (Rha)	20.56	15.03	17.8 (2.8)	16.08	10.84	13.5 (2.6)
Fucose (Fuc)	2.17	0 (ND)	1.1 (1.1)	0 (ND)	0 (ND)	0 (ND)
Xylose (Xyl)	19.88	23.27	21.8 (1.7)	18.99	21.06	20.0 (1.0)
Mannose (Man)	8.74	12.73	10.7 (2.0)	15.56	19.91	17.7 (2.2)
Galactose (Gal)	14.66	16.65	15.7 (1.0)	7.87	13.2	10.5 (2.7)
Glucose (Glc)	0.89	2.29	1.59 (0.7)	2.18	1.48	1.6 (0.7)
N-acetylglucos- amine (GlcNAc)	12.3	2.03	7.2 (5.1)	0 (ND)	0 (ND)	0 (ND)
N-acetylgalacto-samine (GalNAc)	0.35	0 (ND)	0.18 (0.18)	0 (ND)	0 (ND)	0 (ND)
2-N-acetyl-2,6-dideoxy galactose (FucNAc)	+ (*)	0 (ND)	+ (*)	0 (ND)	0 (ND)	0 (ND)
2-N-acetyl-2,6-dideoxy Glucose (QuiNAc)	+ (*)	0 (ND)	+ (*)	0 (ND)	0 (ND)	0 (ND)
L-glycero-D-manno Heptose (L-D-Hep)	3.0	4.31	3.65 (0.7)	0 (ND)	4.03	2.0 (2.0)

LPS from Leptospira interogans serovar Copenhageni strain L1-130 (L1-130) and intermediate pathogenic, Leptospira licerasiae serovar Varillal strain VAR010 (VAR010) were purified from both aqueous (AQ) and phenol layer (PH) and subjected for composition analysis separately. The sugar composition values of aqueous, phenol and combined mean values (± SE) are presented as mole percentage of monosaccharides obtained in fractions. ND = not detected; FucNAc = 2-Nacetyl-2, 6-dideoxy Galactose and QuiNAc = 2-Nacetyl-2, 6-dideoxy Glucose (*). FucNAc and QicNAc are proposed according to the EI mass fragmentation pattern and relative retention times; however quantification could not be done due to unavailability of authentic standards for both monosaccharides

ISSN: 1471-2180