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Fig. 2 | BMC Microbiology

Fig. 2

From: Preferential production of G-CSF by a protein-like Lactobacillus rhamnosus GR-1 secretory factor through activating TLR2-dependent signaling events without activation of JNKs

Fig. 2

Preferential G-CSF production by GR-1 is unique among other bacteria in primary BMDMs. a-b Primary BMDMs were treated with live S. aureus, live E. coli GR-12 or live GR-1 at indicated bacteria/macrophage ratios (CFU/cell). Production of TNF-α (a; in 4 h) and G-CSF (b; in 24 h) was measured from cell culture media using ELISA. [n ≥ 3; p < 0.05 by one-way ANOVA with Tukey’s multiple comparison post hoc test; columns accompanied by the same letter (a, b or c) are not significantly different from each other]. c Primary BMDMs were treated with live GR-1 (20 CFU/cell) or lipoteichoic acid (LTA; 10 μg/ml) for indicated time points. Production of TNF-α (in 4 h) and G-CSF (in 24 h) was measured from cell culture media using ELISA. Data shown as mean ± SEM [n ≥ 3]

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