Multiplex PCR for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics

Table 4 Identification results of representative Vibrio isolates between Vibrio multiplex PCR and MALDI-TOF MS system

Identification of Vibrio isolates by Vibrio multiplex PCR		Identification of Vibrio isolates by MALDI-TOF MS analysis
Lane No. in Fig. 2 ^a	Description	MALDI-TOF MS results	log score value^b
1	Vibrio alginolyticus	Vibrio alginolyticus DSM 2171 T	1.709
2	Vibrio spp.	Vibrio mytili DSM 19137 T	1.721
3	Vibrio alginolyticus	Vibrio alginolyticus DSM 2171 T	1.812
4	Vibrio parahaemolyticus	Vibrio parahaemolyticus DSM 11058	1.975
5	Vibrio spp.	Vibrio mytili DSM 19137 T	1.756
6	Vibrio alginolyticus	Vibrio alginolyticus DSM 2171 T	1.761
7	Vibrio spp.	Vibrio fortis DSM 19133 T	1.933
8	Vibrio vulnificus	Vibrio vulnificus DSM 10143 T	2.06
9	Vibrio alginolyticus	Vibrio alginolyticus CCM 2578 T	1.755
10	Vibrio parahaemolyticus	Vibrio parahaemolyticus DSM 11058	1.951
11	Vibrio vulnificus	Vibrio vulnificus CCUG 38429	2.144

^aThe number indicate lane number of Vibrio isolates in Fig. 2
^bIsolates identification (log) score values, >2.0 indicated species-level, between 1.7 and 2.0 indicated genus level identification, respectively

ISSN: 1471-2180