Multiplex PCR for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics

Table 3 Number of isolated Vibrio strains from local areas of Korea and their multiplex PCR results

Area of sampling	# of sample	Results of each specific primer set in multiplex PCR^a
		VP	VC	VV	VA	VM	Vibrio genus
		VP C1155272 F-R	VC C634002 F-R	VV 2055918 F79-R	VA 1198230 F-R	VM C727581 F-R	VG C2694352 F46-R734
Yeosu (by MFDS^b)	20			20			20
Busan	8	2		5	1		8
Busan (by BMIHE^c)	34	34					34
Geoje	14				4		14
Jinhae	34	5			21		34
Chungmu	7				2		7
Total	117	41	0	25	28	0	117

^aVP, V. parahaemolyticus; VC, V. cholerae; VV, V. vulnificus; VA, V. alginolyticus; VM, V. mimicus
^bMFDS, Ministry of Food and Drug Safety in South Korea
^cBMIHE, Busan Metropolitan city Institute of Health & Environment

ISSN: 1471-2180