Skip to main content

Table 2 Primer pairs for Vibrio multiplex PCR designed and used in this study and their sources

From: Multiplex PCR for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics

Primer name Source of genea Target genus or species PCR product size (bp) Final primer Conc. (μM) Primer sequences (5’-3’)b Protein of target gene
VP 1155272 F NC_004605.1 (c1155272-1154856) Vibrio parahaemolyticus 297 0.24 5’ AGCTT ATTGG CGGTT TCTGT CGG hypothetical protein VPA1095
VP 1155272 R 5’ CKCAA GACCA AGAAA AGCCG TC
VC C634002 F NC_002506.1 (c634002-633547) Vibrio cholerae 154 0.24 5’ CAAGC TCCGC ATGTC CAGAA GC hypothetical protein VCA0694
VC C634002 R 5’ GGGGC GTGAC GCGAA TGATT
VV 2055918 F79 NC_005139.1 (2055918–2056664) Vibrio vulnificus 484 0.4 5’ CAGCC GGACG TCGTC CATTT TG hypothetical protein VV2055
VV 2055918 R 5’ ATGAG TAAGC GTCCG ACGCG T
VA 1198230 F NZ_CH902589.1 (1198230–1198616) Vibrio alginolyticus 199 0.1 5’ ACGGC ATTGG AAATT GCGAC TG whole genome shotgun sequence
VA 1198230 R 5’ TACCC GTCTC ACGAG CCCAA G
VM C727581F NZ_ADAF01000001.1 (c727581-726859) Vibrio mimicus 249 0.8 5’ ATAAA GCGGG CTTGC GTGCA contig43, whole genome shotgun sequence
VM C727581R 5’ GATTT GGRAA AATCC KTCGT GC
VG C2694352 F46 NC_004603.1 (c2694352-2693309) Vibrio genus 689 1 5’ GTC ARA TTG AAA ARC ART TYG GTA AAG G recombinase A
VG C2694352 R734 5’ ACY TTR ATR CGN GTT TCR TTR CC
  1. aReference sequence number of chromosomes in GenBank at the NCBI and position of gene
  2. bMixed base: K = G + T; R = A + G; Y = C + T; N = A + C + G + T