Table 2 Primer pairs for Vibrio multiplex PCR designed and used in this study and their sources
Primer name | Source of genea | Target genus or species | PCR product size (bp) | Final primer Conc. (μM) | Primer sequences (5’-3’)b | Protein of target gene |
|---|---|---|---|---|---|---|
VP 1155272 F | NC_004605.1 (c1155272-1154856) | Vibrio parahaemolyticus | 297 | 0.24 | 5’ AGCTT ATTGG CGGTT TCTGT CGG | hypothetical protein VPA1095 |
VP 1155272 R | 5’ CKCAA GACCA AGAAA AGCCG TC | |||||
VC C634002 F | NC_002506.1 (c634002-633547) | Vibrio cholerae | 154 | 0.24 | 5’ CAAGC TCCGC ATGTC CAGAA GC | hypothetical protein VCA0694 |
VC C634002 R | 5’ GGGGC GTGAC GCGAA TGATT | |||||
VV 2055918 F79 | NC_005139.1 (2055918–2056664) | Vibrio vulnificus | 484 | 0.4 | 5’ CAGCC GGACG TCGTC CATTT TG | hypothetical protein VV2055 |
VV 2055918 R | 5’ ATGAG TAAGC GTCCG ACGCG T | |||||
VA 1198230 F | NZ_CH902589.1 (1198230–1198616) | Vibrio alginolyticus | 199 | 0.1 | 5’ ACGGC ATTGG AAATT GCGAC TG | whole genome shotgun sequence |
VA 1198230 R | 5’ TACCC GTCTC ACGAG CCCAA G | |||||
VM C727581F | NZ_ADAF01000001.1 (c727581-726859) | Vibrio mimicus | 249 | 0.8 | 5’ ATAAA GCGGG CTTGC GTGCA | contig43, whole genome shotgun sequence |
VM C727581R | 5’ GATTT GGRAA AATCC KTCGT GC | |||||
VG C2694352 F46 | NC_004603.1 (c2694352-2693309) | Vibrio genus | 689 | 1 | 5’ GTC ARA TTG AAA ARC ART TYG GTA AAG G | recombinase A |
VG C2694352 R734 | 5’ ACY TTR ATR CGN GTT TCR TTR CC |