A multilocus sequence analysis scheme for characterization of Flavobacterium columnare isolates

BMC Microbiology

Table 2 Proportion of isolates of F. columnare from each region (south and north) and year of isolation within the MLSA clusters (%)

MLSA clusters	No. of isolates	North	South	P value	2003	2005	2006	2007	2008	2009	2010	2011	2012	P value
C	34	58.8	41.2	0.182	0	0	23.5	14.7	8.8	20.6	17.6	2.9	11.8	0.88
E	20	0	100	-	5.0	0	0	10.0	15.0	0	25.0	5.0	40.0	0.003^*
G	14	57.1	42.9	0.593	0	14.3	7.1	35.7	14.3	7.1	21.4	0	0	0.145
H	3	33.3	66.7	0.564	66.7	0	0	0	0	0	0	0	33.3	-^a
A	12	50	50	1.00	9.1	0	18.2	27.3	0	27.3	18.2	0	0	0.118

Observations for regional sources were compared by nonparametric chi-square test (* = P < 0.05). Observations for year of isolation were tested with logistic regression where year was fitted as a continuous covariate
^aDue to the small number of cluster H isolates in our dataset (n = 3), we did not include cluster H in logistic regression

ISSN: 1471-2180