A multilocus sequence analysis scheme for characterization of Flavobacterium columnare isolates

BMC Microbiology

Table 1 Characteristics of the six loci used in the analyses for the 83 F. columnare strains

Locus	Fragment size(bp)	No. of alleles	Allelic diversity	GC	S	dN/dS	∏	Tajima’s D
trpB	626	5	0.72	0.441	49	0.046	0.0283	2.45^a
rpoD	386	3	0.53	0.348	10	0.000	0.0117	3.38^a
tuf	608	4	0.29	0.399	3	0.126	0.0005	−0.85^b
atpA	645	4	0.56	0.425	3	0.000	0.0015	0.52^b.
dnak	643	2	0.27	0.362	1	0.000	0.0004	0.43^b.
gyrB	598	3	0.37	0.369	2	0.000	0.0006	−0.07^b.
Concatenated	3509	9	0.74	0.390	68	-	-	-
Average	-	-	0.4604	-	-	-	-	-

GC Guanine + Cytosine content; S number of segregating sites; dN/dS the ratio of non-synonymous to synonymous substitutions; ∏ nucleotide diversity; Tajima’s D neutrality test values
^astatistically significant, and ^bnon-significant value

ISSN: 1471-2180