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Table 2 Strains isolated from different environments, their molecular identification and biosurfactant production and other characteristics

From: Exploiting the aerobic endospore-forming bacterial diversity in saline and hypersaline environments for biosurfactant production

Strains Isolation media Identification (% identity) – accession number a BOX group E24-Hex E24-Ker DC OD (cm) H sfp AMS
(EI %) (EI %)
1 day 15 days 30 days 1 day 15 days 30 days
Vermelha Lagoon               
 VM 1.0 MB Bacillus aquimaris (99) - JF411268.1   27 ± 2.3 - - - - - - - - - -
 VM 1.1 MB B. subterraneus (100) - KJ722431.1   - - - - - - - - - - -
 VM 2.0 MB B. infantis (99) - KJ911913.1   - - - - - - - - - + +
 VM 2.1 MB B. infantis (100) - KJ911913.1   10 ± 0 8 ± 0 - 24 ± 5.7 16 ± 8 12 ± 5.7 + - - + +
 VM 3.0 MB Halobacillus trueperi (99) - KJ009504.1   - - - - - - - - - - -
 VM 3.1 MB B. vietnamensis (100) - JF411279.1 14 38 ± 6.9 38 ± 6.9 38 ± 6.9 - - - + - - - +
 VM 4.0 MB H. mangrovi (99) - JQ799099.1   - - - - - - - - - - -
 VM 5.0 MB B. berkeleyi (99) - NR109459.1   - - - - - - - - - - -
 VM 5.1 MB B. berkeleyi (99) - NR109459.1   - - - - - - - - - - -
 VM 6.0 MB B. infantis (99) - KJ911913.1 15 19 ± 2.3 15 ± 1.2 15 ± 1.2 34 ± 2.8 27 ± 9.2 13 ± 8.3 - - - + +
 VM 6.1 MB B. infantis (99) - KJ911913.1 16 47 ± 5.8 24 ± 6.9 21 ± 6.1 8 ± 83.5 6 ± 0 - - - - - -
 VM 7.0 MB H. trueperi (99) - KJ009504.1   - - - - - - - - - + -
 VM 7.1 MB Thalassobacillus hwangdonensis (100) - NR104552.1   - - - - - - - - - - -
 VM 8.0 MB B. infantis (99) - KJ911913.1 9 19 ± 1.2 19 ± 1.2 15 ± 1.2 30 ± 2.8 17 ± 1.2 11 ± 1.2 - - - - -
 VM 8.1 MB B. infantis (99) - KJ911913.1   21 ± 1.2 21 ± 1.2 21 ± 1.2 - - - + - + - -
 VM 9.0 MB H. mangrove (99) - JQ799099.1   - - - - - - - - - + -
 VM 10 TSB + 3.5 % NaCl B. subterraneus (99) - KJ722431.1   - - - - - - - - - + -
 VM 20 TSB + 3.5 % NaCl B. infantis (99) - KJ911913.1 17 18 ± 3.5 12 ± 4 12 ± 4 32 ± 5.7 - - - - - - +
 VM 30 TSB B. infantis (99) - KJ911913.1 4 33 ± 2.3 33 ± 2.3 30 ± 0 36 ± 2.8 28 ± 5.7 20 ± 5.7 + - - - -
 VM 40 TSB B. infantis (99) - KJ911913.1 10 23 ± 2.3 23 ± 2.3 23 ± 2.3 36 ± 2.8 31 ± 9.5 31 ± 9.5 - - - - -
 VM 41 TSB B. infantis (99) - KM077280.1 4 17 ± 6.1 16 ± 7.2 16 ± 7.2 32 ± 2.8 24 ± 3.5 22 ± 3.5 - - - - -
 VM 50 TSB B. licheniformis (100) - LN774355.1 12 - - - 31 ± 2.3 - - - - + - +
 VM 60 TSB Paenibacillus campinasensis (99) - JF830004.1   - - - - - - - - - - -
 VM 70 TSB + 7 % NaCl T. devorans (100) - JQ799100.1   - - - - - - - - - - -
 VM 80 TSB + 3.5 % NaCl B. infantis (99) - KM077280.1 18 13 ± 1.2 13 ± 1.2 13 ± 1.2 38 ± 3.5 29 ± 8.3 19 ± 8.1 + - - - -
 VM 81 TSB + 3.5 % NaCl B. infantis (99) - KJ911913.1 4 54 ± 7.2 47 ± 4.6 42 ± 3.5 27 ± 5.8 25 ± 4.6 19 ± 2.3 - - - - -
Dois Rios Beach               
 DR 1.0 TSB B. niacini (99) - KJ542777.1   - - - - - - - - - - -
 DR 2.0 TSB B. asahii (99) - JN084129.1 7 - - - 32 ± 2.8 19 ± 9 14 ± 3.5 - - - - -
 DR 3.0 MB Fictibacillus barbaricus (99) - KJ575018.1   - - - - - - - - - - -
 DR 4.0 MB F. barbaricus (99) - KC778367.1 6 63 ± 2.3 63 ± 2.3 61 ± 1.2 - - - - - + - -
Massambaba Beach               
 MA 1.0 MB B. subtilis (99) - KJ668821.1 1 - - - 56 ± 6.4 46 ± 7.8 43 ± 4.2 + 2.3 + + -
 MA 2.0 MB B. aquimaris (99) - EU624438.1 8 - - - 56 ± 2.1 31 ± 5.2 30 ± 3.5 - - - + -
 MA 2.1 MB B. aquimaris (99) - KM677908.1   - - - - - - - - + + -
 MA 3.0 MB B. subtilis (100) - KP100527.1 1 - - - 54 ± 3 46 ± 4.6 46 ± 4.6 + 2 + + +
 MA 3.1 MB B. subtilis (100) - KP100527.1 3 28 ± 4.9 25 ± 1.7 24 ± 1.2 45 ± 0 44 ± 1.7 40 ± 5.2 - 1.5 + + +
 MA 5.0 MB B. subtilis (100) - KP100527.1 2 34 ± 6 34 ± 6 32 ± 3.5 - - - + 2.5 + + -
 MA 5.1 MB B. tequilensis (99) - KC851826.1 1 48 ± 5.5 26 ± 2.9 26 ± 2.9 27 ± 1.7 17 ± 6 17 ± 6 + 1.5 + + -
 MA 6.0 MB B. aquimaris (100) - KM677908.1   - - - - - - - - - - -
 MA 6.1 MB B. aquimaris (99) - KM677908.1   14 ± 0 13 ± 1.7 10 ± 3.5 - - - - - + + -
 MA 7.0 MB B. subtilis (99) - KP100527.1 3 26 ± 2.9 - - 48 ± 4.2 41 ± 13.8 35 ± 9.2 + 1.7 + + -
 MA 7.1 MB B. subtilis (99) - KJ668821.1 1 36 ± 3.5 30 ± 3.5 30 ± 3.5 13 ± 1.7 - - + 3 + + -
 MA 8.0 TSB B. subtilis (99) - KP100527.1 3 30 ± 2.1 27 ± 1.7 25 ± 2.9 18 ± 4.6 14 ± 7.9 11 ± 5.2 + 1.7 + + -
 MA 9.0 MB B. aquimaris (100) -KM677908.1   - - - - - - - - - + -
 MA 9.1 MB B. aquimaris (100) -KM677908.1   - - - - - - - - + + -
 MA 10.0 MB B. subtilis (100) -KP100527.1 1 9 ± 1.7 8 ± 0 - 45 ± 4.6 44 ± 3.5 35 ± 1.7 + 1.9 + + -
 MA 10.1 MB B. subtilis (100) -KP100527.1 1 - - - 55 ± 1.7 49 ± 7.1 46 ± 4.6 + 1.8 + + -
 MA 11.0 MB B. subtilis (99) -KP100527.1 3 - - - 46 ± 2.9 38 ± 8.7 34 ± 6 + 3.1 + + -
 MA 11.1 MB B. subtilis (99) -KP100527.1 3 - - - 44 ± 1.2 44 ± 1.7 30 ± 1.7 + 2 + - -
 MA 12.0 MB B. subtilis (99) -KF601955.1   - - - 14 ± 0 7 ± 4 7 ± 4 + 2.5 + + -
 MA 12.1 MB B. subtilis (100) -KP100527.1 2 - - - 54 ± 3 50 ± 3.5 44 ± 1.7 + 1.6 + + -
 MA 13.0 MB B. subtilis (100) KP100527.1 2 - - - 51 ± 0 25 ± 5.5 19 ± 6.9 + 2.0 + + -
 MA 13.1 MB B. tequilensis (99) - KC851826.1 1 4 ± 1.2 - - 53 ± 4.6 48 ± 5.5 44 ± 1.7 + 2.7 + + -
 MA 14.0 MB B. subtilis (100) KP100527.1 3 8 ± 0 8 ± 0 - 46 ± 2.9 32 ± 3.5 28 ± 0 + 2.5 + + -
 MA 14.1 MB B. subtilis (100) KP100527.1 3 - - - 53 ± 1.7 45 ± 0 44 ± 1.7 + 1.1 + + -
 MA 15.0 TSB + 3.5 % NaCl B. subtilis (100) KP100527.1 1 36 ± 7.5 31 ± 6 31 ± 6 18 ± 3.5 12 ± 4.6 12 ± 4.6 + 2 + + -
 MA 15.1 TSB + 3.5 % NaCl B. licheniformis (100) - KP052704.1 11 32 ± 3.5 25 ± 2.9 23 ± 0 22 ± 4.6 22 ± 4.6 23 ± 5.2 + 1.6 + + +
 MA 16.0 TSB + 3.5 % NaCl B. tequilensis (99) - KC851826.1 1 20 ± 0 20 ± 0 19 ± 1.7 51 ± 4.2 48 ± 4.2 43 ± 4.2 + 2.2 + + -
 MA 16.1 TSB + 3.5 % NaCl B. subtilis subsp. subtilis (99) – KM972669.1 3 12 ± 3.5 12 ± 3.5 11 ± 4.6 46 ± 4.6 26 ± 7.1 18 ± 6.9 + 2.6 + + -
Abraão Beach               
 AB 1.0 TSB + 3.5 % NaCl B. subtilis subsp. subtilis (99) – KM972669.1   - - - - - - + 1.8 + + -
 AB 1.1 TSB + 3.5 % NaCl B. subtilis subsp. subtilis (99) – KM972669.1 3 14 ± 0 10 ± 3.5 10 ± 3.5 51 ± 0 37 ± 4.2 29 ± 3.5 + 2.5 + + -
 AB 2.0 TSB + 3.5 % NaCl B. subtilis (100) KP100527.1 1 46 ± 2.9 32 ± 1.7 28 ± 0 59 ± 1.7 53 ± 3.5 53 ± 3.5 + 2 + + +
 AB 3.0 TSB + 3.5 % NaCl B. aquimaris (100) - KM516787.1   - - - - - - - - - - -
 AB 4.0 TSB + 3.5 % NaCl B. aquimaris (99) - KM516787.1   - - - 19 ± 1.7 - - - - - - -
Saltern 1               
 SA1 1 TSB F. barbaricus (100) - KJ831620.1   - - - - - - - - + + -
 SA1 2 TSB + 7 % NaCl F. barbaricus (100) - KJ831620.1   - - - - - - - - + - -
 SA1 3 TSB + 7 % NaCl F. barbaricus (100) - KJ831620.1   - - - - - - - - - - -
 SA1 4 TSB + 7 % NaCl F. barbaricus (98) -KJ575018.1   - - - - - - - - - - -
 SA1 5 MB F. barbaricus (100) - KJ831620.1   - - - - - - - - - - -
 SA1 6 MB B. oceanisediminis (100) - KM873030.1   - - - - - - - - + - -
 SA1 7 MB B. firmus (99) - KP119100.1   - - - - - - - - - - -
 SA1 8 MB B. firmus (99) - KF886286.1   - - - - - - - - - - -
 SA1 10 MB B. firmus (99) - KP119100.1 2 32 ± 3.5 - - 49 ± 1.7 - - - - + - -
 SA1 11 MB F. barbaricus (100) - KJ831620.1   - - - - - - - - + - -
 SA1 12 MB F. barbaricus (99) - KJ831620.1   - - - - - - - - + - -
 SA1 13 MB F. barbaricus (100) - KJ831620.1   - - - - - - - - + - -
 SA1 14 MB B. firmus (100) -KP119100.1 2 - - - 51 ± 7.4 - - - - + - -
 SA1 15 MB B. firmus (100) - KJ528874.1 2 - - - 52 ± 7.1 - - - - + - -
 SA1 16 MB B. firmus (99) - KF886286.1 2 38 ± 3.5 38 ± 3.5 14 ± 0 52 ± 1.7 13 ± 1.7 - - - - - -
 SA1 17 MB B. firmus (99) - KP119100.1 2 43 ± 2.9 - - 48 ± 0 17 ± 5.2 - - - - - -
 SA1 18 TSB + 7 % NaCl B. firmus (99) - KP119100.1 2 41 ± 1.7 14 ± 0 - 46 ± 2.9 15 ± 1.7 - - - - - -
 SA1 19 TSB + 7 % NaCl B. niabensis (99) -KF861607.1   - - - - - - - - - - -
Saltern 2               
 SA2 1 MB B. oceanisediminis (100) - KM873030.1 5 39 ± 1.7 14 ± 0 - 51 ± 4.2 - - - - - - -
 SA2 2 MB B. firmus (100) - KP119100.1 5 36 ± 1.7 7 ± 4 7 ± 4 50 ± 6.4 - - - - - - -
 SA2 3 MB B. firmus (100) - KP119100.1 2 - - - 65 ± 2.9 44 ± 10 38 ± 6.9 - - - - -
 SA2 4 MB B. firmus (100) - KP119100.1 5 - - - 51 ± 7.4 43 ± 4.6 42 ± 3.5 - - - - -
 SA2 5 MB B. firmus (100) - KP119100.1 2 - - - 51 ± 4.2 - - - - - - -
 SA2 6 MB B. firmus (100) - KP119100.1   - - - - - - - - - - -
 SA2 7 MB B. firmus (100) - KP119100.1 2 - - - 51 ± 8.5 38 ± 7 - - - - - -
 SA2 8 MB B. oceanisediminis (100) - KM873030.1 2 45 - - - - - - - - - -
 SA2 9 MB B. oceanisediminis (99) - KM873030.1 2 23 ± 8.1 - - 51 ± 7.4 48 ± 4.6 - - - - - -
Saltern 2 (mud)               
 LS 1 TSB + 7 % NaCl B. horikoshii (100) - KJ722424.1   - - - - - - - - - - -
 LS 2 TSB + 7 % NaCl B. horikoshii (100) - KJ722424.1   - - - - - - - - - - -
 LS 3 TSB + 7 % NaCl B. horikoshii (100) - KJ722424.1   - - - - - - - - - - -
 LS 4 TSB + 7 % NaCl B. horikoshii (100) - KJ722424.1   - - - - - - - - + - -
 LS 5 TSB + 7 % NaCl B. horikoshii (99) - KJ719363.1   - - - - - - - - - - -
 LS 6 MB B. vietnamensis (99) - JF411279.1   - - - - - - - - + - -
 LS 7 MB B. vietnamensis (99) - JF411279.1   - - - - - - - - - - -
 LS 8 MB B. vietnamensis (100) - JF411279.1   - - - - - - - - - - -
 LS 10 MB B. vitnamensis (99) - JF411279.1   - - - - - - - - + - -
 LS 11 MB B. foraminis (97) - KF724906.1   - - - - - - - - - - -
 LS 12 TSB B. foraminis (99) - KF724906.1   - - - - - - - - - - -
 LS 13 TSB Paenisporosarcina indica (99) - NR108473.1 13 - - - 59 ± 7.1 38 ± 7 - - - - - -
 LS 14 TSB P. quisquiliarum (99) - JF309238.1   - - - - - - - - - - -
 LS 15 TSB P. quisquiliarum (99) - JF309238.1   - - - - - - - - - - -
 LS 16 TSB B. licheniformis (100) - LN774355.1   - - - - - - - - - - +
 LS 17 TSB + 3.5 % NaCl B. aquimaris (99) - JF411268.1   - - - - - - - - - - -
 LS 18 TSB + 3.5 % NaCl P. indica (99) - NR108473.1   - - - - - - - - - - -
 LS 19 TSB + 3.5 % NaCl B. thioparans (99) - KJ911915.1   - - - - - - - - - - -
 LS 20 TSB + 3.5 % NaCl B. foraminis (99) -KF724906.1   - - - - - - - - - - -
 LS 21 TSB + 3.5 % NaCl B. foraminis (99) -KF724906.1   - - - - - - - - - - -
  1. a First hit in GenBank with a known species; EI emulsification index, Hex hexadecane, Ker kerosene, DO drop collapse, OD oil displacement, H hemolysis, sfp presence of sfp gene, AMS production of an antimicrobial substance. (−) negative results. Strains in bold are those showing emulsification values higher than 30 %