Table 1 Selected primers with target regions and experimental setup
From: Quinolone resistance mutations in the faecal microbiota of Swedish travellers to India
Gene | Target region | Product size | Forward primer | Reverse primer | Annealing T |
|---|---|---|---|---|---|
gyrA | 129–439 | 311 bp | ggtacaccgtcgcgtacttt | caacgaaatcgaccgtctct | 57 °C |
parC | 20–306 | 287 bp | gccttgcgctacatgaattt | accatcaaccagcggataac | 57 °C |