Microbial inoculants for the biocontrol of Fusarium spp. in durum wheat

BMC Microbiology

Table 7 List of primers for detection of genes belongs to pln locus and LPs shyntetase

Target gene	Primers	Annealing temperature (°C)	References
plnE/F	fw: 5′- GGCATAGTTAAAATTCCCCCC-3′	53.2	Doulgeraki et al., 2013 [37]
plnE/F	rev: 5′- CAGGTTGCCGCAAAAAAAG-3′	53.2	Doulgeraki et al., 2013 [37]
plnJ	fw: 5′-TAACGACGGATTGCTCTG-3′	51	Doulgeraki et al., 2013 [37]
plnJ	rev: 5′- AATCAAGAAATTATCACATTAGTC-3′	51	Doulgeraki et al., 2013 [37]
plnK	fw: 5- CTGTAAGCATTGCTAACCAATC	52.9	Doulgeraki et al., 2013 [37]
plnK	rev: 5- ACTGCTGACGCTGAAAAG	52.9	Doulgeraki et al., 2013 [37]
plnG	fw: 5- TGCGGTTATCAGTATGTCAAAG	52.8	Doulgeraki et al., 2013 [37]
plnG	rev: 5-CCTCGAAACAATTTCCCCC	52.8	Doulgeraki et al., 2013 [37]
plnN	fw: 5- ATTGCCGGGTTAGGTATCG	51.9	Doulgeraki et al., 2013 [37]
plnN	rev: 5- CCTAAACCATGCCATGCAC	51.9	Doulgeraki et al., 2013 [37]
Surfactin synthetase	As1-f CGCGGMTACCGVATYGAGC	43 °C	Tapi et al., 2010 [38]
Surfactin synthetase	Ts2-r ATBCCTTTBTWDGAATGTCCGCC	43 °C	Tapi et al., 2010 [38]
Fengycin synthetase	Af2-f GAATAYMTCGGMCGTMTKGA	45 °C	Tapi et al., 2010 [38]
Fengycin synthetase	Tf1-r GCTTTWADKGAATSBCCGCC	45 °C	Tapi et al., 2010 [38]
Mycosubtilins syntetase	Am1-f CAKCARGTSAAAATYCGMGG	45 °C	Tapi et al., 2010 [38]
Mycosubtilins syntetase	Tm1-r CCDASATCAAARAADTTATC	45 °C	Tapi et al., 2010 [38]
Iturin A synthetase	ituC-f AAAGGATCCAAGCGTGCCTTTTACGGGAAA	56 °C	Alvarez et al. 2011 [39]
Iturin A synthetase	ituC-r AAAAAGCTTAATGACGCCAGCTTTCTCTT	56 °C	Alvarez et al. 2011 [39]

ISSN: 1471-2180