Primera | Target gene | Nucleotide sequence (5' → 3')b | Product length (bp) |
---|---|---|---|
Gene specific primer pairs | |||
bcrC_108_F | bcrC | CAAGTGGTGGCAACGATGTGT | 191 |
bcrC_299_R | GAAGGTCTGGCGATACTGGATGC | ||
pchF_1336_F | pchF | GGCCGGCAACGTCATCATC | 273 |
pchF_1099_R | CCATCCGGGAGCACCACT | ||
hped_153_F | hped | TGATCGAAGGCAAGGGCGGAAAAG | 331 |
hped _473_R | GCGGCGGTGTAGGGCGTGATG | ||
acsA_1373_F | acsA | GCCGCGGTGAGGTT | 306 |
acsA_1068_R | CGGGGTGAATGTCCA | ||
etpR_425_F | etpR | AATTGGCCGCTCTTCTG | 310 |
etpR_116_R | TTTCGGCATGTTTGTCA | ||
etpR_1717_Fc | etpR | TGGCGACGGCATTCTC | 219 |
etpR_1499_Rc | TGCCGCATCTGTTCACC | ||
ebA326_41_F | ebA326 | TGGCTGGATCTCTGCTC | 275 |
ebA326_315_R | TTCCCGTGCGACCTG | ||
ebA327_1070_F | ebA327 | GCTTCGCGGTCCTGA | 375 |
ebA327_1480_R | TGTCGCGGTTGTAGC | ||
ebA329_212_F | ebA329 | TGCGGCCCCTGATG | 316 |
ebA329_537_R | ACGATGCCGCTGTGG | ||
ebA332_488_F | ebA332 | CCGGCGTGGAGGTAG | 285 |
ebA332_772_R | GGCGCGGGGTTTT | ||
ebA335_1092_F | ebA335 | GCTGGGGGAGACGAA | 253 |
ebA335_1344_R | CGCCGCCTTGTTGT | ||
Generation of Δ etpR deletion mutation | |||
acsA_BamHI_F | acsA | AAGGATCCCACGAAATGTCTCCTGAACCCTGC | 1300 |
acsA_SphI_R | ACCGGGCATGCGCCCACCAGC | ||
ebA327_BamHI_F | ebA326/ebA327 | GATCAGGATCCACGTCACCG | 2350 |
ebA326_BamHI_R | AAGGATCCTGACCGTCGGAGGACCGGATAGATC | ||
Identification of Δ etpR genotype | |||
ΔetpR _F | 3'-IR-etpR f | TGGGCGTAGCGTAGT | 2133d/ 318e |
ΔetpR _R | 5'-IR-etpR f | TGGATTGTTCTGTAT | |
Generation of in trans complementation of etpR | |||
etpR_XhoI_F | etpR | AACTCGAGCCCACTCCAAGCGTCGAAACACCGGC | 2436 |
etpR_KpnI_R | AAAGGTACCGCTTCGCTCCGGGAAACCAGTGTGCGC |