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Fig. 2 | BMC Microbiology

Fig. 2

From: The predicted σ54-dependent regulator EtpR is essential for expression of genes for anaerobic p-ethylphenol and p-hydroxyacetophenone degradation in “Aromatoleum aromaticum” EbN1

Fig. 2

Physiological characterization of strains. Growth of "A. aromaticum" EbN1 wild type (circles), ΔetpR mutant (triangles) and etpR-complemented (genotype: ΔetpR, pBBR1MCS-2 ΩetpR) mutant (squares) under nitrate-reducing conditions with p-ethylphenol (blue, a), benzoate (green, b), p-hydroxyacetophenone (red, c) and a binary mixture of benzoate and p-hydroxyacetophenone (d). Cultures were inoculated with cells adapted to anaerobic growth with benzoate. All growth experiments were performed in duplicates with high reproducibility; for each experiment one representative experiment is depicted. The wild type harboring pBBR1MCS-2 showed wild type-like growth with p-hydroxyacetophenone. In contrast, the ∆etpR mutant with pBBR1MCS-2, like the ∆etpR mutant, was unable to utilize p-hydroxyacetophenone (data not shown). Optical density: black, filled symbols; substrate concentrations: colored, open symbols

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BMC Microbiology

ISSN: 1471-2180

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