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Fig. 1 | BMC Microbiology

Fig. 1

From: The predicted σ54-dependent regulator EtpR is essential for expression of genes for anaerobic p-ethylphenol and p-hydroxyacetophenone degradation in “Aromatoleum aromaticum” EbN1

Fig. 1

Generation of ΔetpR deletion and etpR-complemented mutants. Scale model of enlarged section of the "p-ethylphenol" gene clusters on the chromosome of "A. aromaticum" EbN1, displaying etpR and its 3'- and 5'-neighbouring regions in the wild type (top) and the ΔetpR mutant (bottom). The chromosomal hybridization location of primer pairs used for construction of the knockout vector pK19 ΩacsAebA326/7 and the complementation plasmid pBBR1MCS-2 ΩetpR are shown in grey (Tables 2 and 3). The positions of the primer pair ΔetpR_F/R for identifying the knockout genotype and an etpR-specific primer pair are indicated in black and the lengths of the corresponding PCR products are given below the gene model (a). Electropherogram of PCR products obtained from the wild type, the ΔetpR mutant and the etpR-complemented mutant applying different primer pairs. Using the primer pair ΔetpR_F/R, a 2,133 bp long amplicon was obtained for the chromosome of the wild type and a shorter, 318 bp long, amplicon for the ΔetpR mutant and the etpR-complemented mutant. Accordingly, amplification of etpR (310 bp) was not possible for the ΔetpR mutant, but for the etpR-complemented mutant (b). Abbreviations: ΔetpR, ΔetpR mutant; etpR-compl., etpR-complemented mutant

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