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Table 1 Partial alignment of fungal di-TPS highlighting catalytic domains

From: Molecular and functional evolution of the fungal diterpene synthase genes

Fungus Sequence name Mg2+-PPi binding (D/E enriched region after an alpha helix) H+-initiated cyclization (DXDD) Mg2+-ionization/cyclization DEXXE
A. take_a RDQLGQWHDVEKSNHIGVEL APHTADVDDTAKALLAL MLSMFGYQCDEFIETVAGPA
C. paspali_a QQQLSEWNDVEITNHIGVEL APHTADVDDTAKALLAL MLSLLGYQTDEFIETVAGSA
F. fujikuroi_a KRQLAVWNDVEDTNHIGVEF APRTADVDDTAKALLAL YLSLLGYQTDEYMEAVAGPV
F. proliferatum_a KRQLAVWKDVEETNHIGVEL APRTADVDDTAKALLAL YLSLLGYQTDEYMEAVAGPV
C. purpurea_b QLQLSQWDDVEKSNHIGVEL APHTADVDDTAKALLAL MLSLLGYQTDEFIEAVAGPA
M. oryzae_b RTQLQNWDDVLSTNHIGIEI APRAPDADDTAKGLLAL LVSMLSYQADEFIHKSAAPA
P. nodorum_a EEQLIAWDDVLDTNHIGVEM APRAVDVDDTAKGLLTL IISMLGYQIDEFFEAEAAPA
S. manihoticola_a VSQLQQWDDLVESNHIGVEL APRTADVDDTAKGLLAL LLSMYGYQNDEFFETHAMAG
M. graminicola_a QSQLATWTDMEATNHIGVEL APGTVDVDDTAKGLLAM FMTLINIQVDEFIEGIATQA
B. sorokiniana_a DRQLNAWNDLVSTNHIGVEL VQRAVDVDDTAKGLLAL VMSLLGFQTDEFFEAFATPA
B. maydis_a DRQLNAWNDLVSTNHIGVEL IQRAVDVDDTAKGLLAL VMSLIGFQTDEFFEAFATPA
A. benhamiae_a RSQLEEWRVSETMHVGFEII PDFQSDMDDTARGMIAL IISFLNYQADEFLEAVAGPH
T. verrucosum_a RSQLEEWRVSETMHVGFEII PDFQSDMDDTARGMIAL IISFLNYQADEFLEAVAGPH
T. equinum_a RSQLEEWRVSETMVGFEIIV PDFQPDMDDTARGMIAL VISFLNYQADEFLEAVAGPQ
T. rubrum_a RSQLEEWRVSETMHVGFEII PDFQSDMDDTARGMIAL IISFLNYQADEFLEAVAGPH
T. tonsurans_a RSQLEEWRVSETMHVGFEII PDFQPDMDDTARGMIAL VISFLNYQADEFLEAVAGPQ
P. amygdali_b RAQLAALDVSTTEHVGFEII PGFQADVDDTAKTISTL FIAMLNFQLDEFMEATAGIL
E. lata_a QRQLQNWDVESSTHVGFEVI PFLQADVDDTAKGLISL VISFLNFQADEFMEATAGPA
E. amarillans_a RHQLLEWDVAATTHVGFEII PHLPTDLDDTAKGIVSL IISFLNYQTDEIMEVVAGAA
E. typhina_a HHQLLEWDVAAATHVGFEII PHLPTDLDDTAKGIVCL IISFLNYQTDEFMEVVAGAA
P. amygdali_a QRQLSDWDIASTAHVGFEII RSISSDADDTAKAAFAL MVSVVDYQADEFMEAVAGLT
M. oryzae_a ERKLSCWNVSETTHVGFEII PSIMADADDTAKTLSAL HISMLSYQGDEYMESVAAPA
P. betae_a HSLLEEWDVSTTNNVGFEII SGIEPDVDDSAKIVTTL ALSFFTYQVDEFMEAVAGPA
A. otae_a KDQLEAWDVASADRVGFEIL CNLEADADDTAKALLAL ILSFLNYQADEYMEAVVGRY
A. nidulans_c SRQLQAWSVKDTVHVGFEII PSIQADADDTAKSLIAV VISFLNYQADEFMEAVVGRL
N. fischeri_a SRELQAWDVKDTVHVGFEMI PSIQADADDTAKALMTV VISFLNYQADEFMEAVVGRL
T. stipitatus_a QAKLETWDVEATDHVGSEIL SGVIADADDTARIILTL MVSMLNFQVDRFIEAVIGQE
P. strigosozonata_a NTALQTWDVKSTERIALEMI ANVGPDSDDTAKALTAL VISMVNFQVDEFFDIVVQKH
S. lacrymans_a VDALQGWDILSTERIAFEMI ANVGPDSDDTAKALTAL VISMVNFQVDEFFDLVVQKH
P. anserina_a RELKAFWLDATTLPVGFEML DDTAKGITTL VISALAYQVDEFIETSVSQL
G. luxurians_a ASQLNSWDVSKAERVGFEVTI MDTGEDADDSSKVLTAL NMCLVTFQIDEFFDSVVQVQ
G. luxurians_b ASQLNSWDVSKAERVGFELTI LDVGEDADDSAKAITAL AITLINFQVDEFFDSMVQDQ
G. luxurians_c ASQLNSWDVSKAERVGFEVTI MDTGEDADDSSKVLTAL NMCLVTFQIDEFFDSVVQVQ
L. palustris_a ERKLEHWDPSACDSVGFEVL AGMMHDADDTAKALQSL VISMLNFQADAYIEKVVGKK
P. teres_a DRQLNAWDVTLTDHVGFEII PYFAPDVDDTSKTITSL TIAMLNFQLDEFMEATAGPA
A. nidulans_a QRLLEAWDLDGTDQVGFEVI PGILADADDTARVLLTL HFSLLVYQVDALMESTAIRM
A. niger_a QQALQQWDVASTLQVGSEVL PGFVPDADDTARALLTQ VISLLNYQVDEYMESVVALL
A. sojae_a SDALNSWNVEATLHVGFEIL PGFVPDADDTARSLLAL VISMLNYQADEYMESVVGSL
T. reesei_a QSALNNWDVNQTLHVGFEML PTLLPDADDTAVSLLAM VISMLNYQVDEYMESVVAYL
R. rufulum_b NRMLKEWNIGSTDRVGFEVL PSCLPDADDTAKTLSAL IISMLGFQVDAYMETVVAEH
G. lozoyensis_a NHMLQNWDIKGSDRVGFEVL SNFLPDADDTAKANTVL VVSMLDYQVDGYMEFVLQEK
A. fumigatus_a QTALKGLDRLLATCTLSVGL PNACADADDTAKALVAL ELSIGLFQEDDLMERSLASL
A. oryzae_a DAALKGLNGLLRTCTLPVSL PKACPDADDTAKALAAL ELSIGIFQEDELMEKSLVNL
A. sojae_b DTALKGLNDLLATCTLPVSV PKACPDADDTAKALAAL ELSIGIFQEDELMEKSLVNL
A. nidulans_b DAALVRLDDLLATSTLTVGL PKACPDADDTAKALIAF ELSVGIFQEDEEMEKSLVNL
P. marneffei_a QKLLDEWNVDSTLHVGFEIL PFVGADADDTATTILVL SISIHTDHSGSYYHRSDWTT
T. stipitatus_b QKLLDDWDVKSTLHVGFEIL PFVGADADDTATTILVL CVSIHTDDSDSYYQRSQWTI
C. purpurea_a   APHTADVDDTAKAILAL MLSLLGYQTDEFIEAVAGPA
M. fijiensis_a TTQLRHCDIESTNHIGVELV GQGATDADDTAKGLLAL FMTLINIQVDEFIEAVITPT
C. globosum_a KEMLHGWDVESTDQVGFEIL PGSLPDVDDTAKGIEAL VLSVLNFHADEYMEGVVEKH
N. crassa_a QSMLLHWDVATTDQVGFELL PGGLVDADDTARAIMAL VLSMLNYQVDEYMETAVERD
N. tetrasperma_a QSMLLTWDVATTDQVGFELL PGGLVDADDTARAIMAL VLSMLNYQVDEYMETAVERD
S. macrospora_a KEMLNKWDVASTDQVGFELL PGGLVDADDTARAIMAL ILSLLNYQVDEYMETAVERD
R. rufulum_a RYLLGCWDASKATPNDLEIL PGSLQDARYIPHAILAL YNDYGSATRDVEEGNLNSLD
B. fuckeliana_a   PGVLPDVDDTSKGLEAL VLSILNFHADEYMEGIIERH
X. parietina_a RRMLSSWDATSSDDVGFEVL PSVLDESDDTGSSIYIL VFSALLYDFDHYMEDIIAGF
Z. passerinii_a ASQLASWNDVEITNHIGVEL PACSRTMMDQHANGSWE YLIRDHSSTMNSRDSA