Table 1 Partial alignment of fungal di-TPS highlighting catalytic domains
From: Molecular and functional evolution of the fungal diterpene synthase genes
Fungus Sequence name | Mg2+-PPi binding (D/E enriched region after an alpha helix) | H+-initiated cyclization (DXDD) | Mg2+-ionization/cyclization DEXXE |
|---|---|---|---|
A. take_a | RDQLGQWHDVEKSNHIGVEL | APHTADVDDTAKALLAL | MLSMFGYQCDEFIETVAGPA |
C. paspali_a | QQQLSEWNDVEITNHIGVEL | APHTADVDDTAKALLAL | MLSLLGYQTDEFIETVAGSA |
F. fujikuroi_a | KRQLAVWNDVEDTNHIGVEF | APRTADVDDTAKALLAL | YLSLLGYQTDEYMEAVAGPV |
F. proliferatum_a | KRQLAVWKDVEETNHIGVEL | APRTADVDDTAKALLAL | YLSLLGYQTDEYMEAVAGPV |
C. purpurea_b | QLQLSQWDDVEKSNHIGVEL | APHTADVDDTAKALLAL | MLSLLGYQTDEFIEAVAGPA |
M. oryzae_b | RTQLQNWDDVLSTNHIGIEI | APRAPDADDTAKGLLAL | LVSMLSYQADEFIHKSAAPA |
P. nodorum_a | EEQLIAWDDVLDTNHIGVEM | APRAVDVDDTAKGLLTL | IISMLGYQIDEFFEAEAAPA |
S. manihoticola_a | VSQLQQWDDLVESNHIGVEL | APRTADVDDTAKGLLAL | LLSMYGYQNDEFFETHAMAG |
M. graminicola_a | QSQLATWTDMEATNHIGVEL | APGTVDVDDTAKGLLAM | FMTLINIQVDEFIEGIATQA |
B. sorokiniana_a | DRQLNAWNDLVSTNHIGVEL | VQRAVDVDDTAKGLLAL | VMSLLGFQTDEFFEAFATPA |
B. maydis_a | DRQLNAWNDLVSTNHIGVEL | IQRAVDVDDTAKGLLAL | VMSLIGFQTDEFFEAFATPA |
A. benhamiae_a | RSQLEEWRVSETMHVGFEII | PDFQSDMDDTARGMIAL | IISFLNYQADEFLEAVAGPH |
T. verrucosum_a | RSQLEEWRVSETMHVGFEII | PDFQSDMDDTARGMIAL | IISFLNYQADEFLEAVAGPH |
T. equinum_a | RSQLEEWRVSETMVGFEIIV | PDFQPDMDDTARGMIAL | VISFLNYQADEFLEAVAGPQ |
T. rubrum_a | RSQLEEWRVSETMHVGFEII | PDFQSDMDDTARGMIAL | IISFLNYQADEFLEAVAGPH |
T. tonsurans_a | RSQLEEWRVSETMHVGFEII | PDFQPDMDDTARGMIAL | VISFLNYQADEFLEAVAGPQ |
P. amygdali_b | RAQLAALDVSTTEHVGFEII | PGFQADVDDTAKTISTL | FIAMLNFQLDEFMEATAGIL |
E. lata_a | QRQLQNWDVESSTHVGFEVI | PFLQADVDDTAKGLISL | VISFLNFQADEFMEATAGPA |
E. amarillans_a | RHQLLEWDVAATTHVGFEII | PHLPTDLDDTAKGIVSL | IISFLNYQTDEIMEVVAGAA |
E. typhina_a | HHQLLEWDVAAATHVGFEII | PHLPTDLDDTAKGIVCL | IISFLNYQTDEFMEVVAGAA |
P. amygdali_a | QRQLSDWDIASTAHVGFEII | RSISSDADDTAKAAFAL | MVSVVDYQADEFMEAVAGLT |
M. oryzae_a | ERKLSCWNVSETTHVGFEII | PSIMADADDTAKTLSAL | HISMLSYQGDEYMESVAAPA |
P. betae_a | HSLLEEWDVSTTNNVGFEII | SGIEPDVDDSAKIVTTL | ALSFFTYQVDEFMEAVAGPA |
A. otae_a | KDQLEAWDVASADRVGFEIL | CNLEADADDTAKALLAL | ILSFLNYQADEYMEAVVGRY |
A. nidulans_c | SRQLQAWSVKDTVHVGFEII | PSIQADADDTAKSLIAV | VISFLNYQADEFMEAVVGRL |
N. fischeri_a | SRELQAWDVKDTVHVGFEMI | PSIQADADDTAKALMTV | VISFLNYQADEFMEAVVGRL |
T. stipitatus_a | QAKLETWDVEATDHVGSEIL | SGVIADADDTARIILTL | MVSMLNFQVDRFIEAVIGQE |
P. strigosozonata_a | NTALQTWDVKSTERIALEMI | ANVGPDSDDTAKALTAL | VISMVNFQVDEFFDIVVQKH |
S. lacrymans_a | VDALQGWDILSTERIAFEMI | ANVGPDSDDTAKALTAL | VISMVNFQVDEFFDLVVQKH |
P. anserina_a | RELKAFWLDATTLPVGFEML | DDTAKGITTL | VISALAYQVDEFIETSVSQL |
G. luxurians_a | ASQLNSWDVSKAERVGFEVTI | MDTGEDADDSSKVLTAL | NMCLVTFQIDEFFDSVVQVQ |
G. luxurians_b | ASQLNSWDVSKAERVGFELTI | LDVGEDADDSAKAITAL | AITLINFQVDEFFDSMVQDQ |
G. luxurians_c | ASQLNSWDVSKAERVGFEVTI | MDTGEDADDSSKVLTAL | NMCLVTFQIDEFFDSVVQVQ |
L. palustris_a | ERKLEHWDPSACDSVGFEVL | AGMMHDADDTAKALQSL | VISMLNFQADAYIEKVVGKK |
P. teres_a | DRQLNAWDVTLTDHVGFEII | PYFAPDVDDTSKTITSL | TIAMLNFQLDEFMEATAGPA |
A. nidulans_a | QRLLEAWDLDGTDQVGFEVI | PGILADADDTARVLLTL | HFSLLVYQVDALMESTAIRM |
A. niger_a | QQALQQWDVASTLQVGSEVL | PGFVPDADDTARALLTQ | VISLLNYQVDEYMESVVALL |
A. sojae_a | SDALNSWNVEATLHVGFEIL | PGFVPDADDTARSLLAL | VISMLNYQADEYMESVVGSL |
T. reesei_a | QSALNNWDVNQTLHVGFEML | PTLLPDADDTAVSLLAM | VISMLNYQVDEYMESVVAYL |
R. rufulum_b | NRMLKEWNIGSTDRVGFEVL | PSCLPDADDTAKTLSAL | IISMLGFQVDAYMETVVAEH |
G. lozoyensis_a | NHMLQNWDIKGSDRVGFEVL | SNFLPDADDTAKANTVL | VVSMLDYQVDGYMEFVLQEK |
A. fumigatus_a | QTALKGLDRLLATCTLSVGL | PNACADADDTAKALVAL | ELSIGLFQEDDLMERSLASL |
A. oryzae_a | DAALKGLNGLLRTCTLPVSL | PKACPDADDTAKALAAL | ELSIGIFQEDELMEKSLVNL |
A. sojae_b | DTALKGLNDLLATCTLPVSV | PKACPDADDTAKALAAL | ELSIGIFQEDELMEKSLVNL |
A. nidulans_b | DAALVRLDDLLATSTLTVGL | PKACPDADDTAKALIAF | ELSVGIFQEDEEMEKSLVNL |
P. marneffei_a | QKLLDEWNVDSTLHVGFEIL | PFVGADADDTATTILVL | SISIHTDHSGSYYHRSDWTT |
T. stipitatus_b | QKLLDDWDVKSTLHVGFEIL | PFVGADADDTATTILVL | CVSIHTDDSDSYYQRSQWTI |
C. purpurea_a | Â | APHTADVDDTAKAILAL | MLSLLGYQTDEFIEAVAGPA |
M. fijiensis_a | TTQLRHCDIESTNHIGVELV | GQGATDADDTAKGLLAL | FMTLINIQVDEFIEAVITPT |
C. globosum_a | KEMLHGWDVESTDQVGFEIL | PGSLPDVDDTAKGIEAL | VLSVLNFHADEYMEGVVEKH |
N. crassa_a | QSMLLHWDVATTDQVGFELL | PGGLVDADDTARAIMAL | VLSMLNYQVDEYMETAVERD |
N. tetrasperma_a | QSMLLTWDVATTDQVGFELL | PGGLVDADDTARAIMAL | VLSMLNYQVDEYMETAVERD |
S. macrospora_a | KEMLNKWDVASTDQVGFELL | PGGLVDADDTARAIMAL | ILSLLNYQVDEYMETAVERD |
R. rufulum_a | RYLLGCWDASKATPNDLEIL | PGSLQDARYIPHAILAL | YNDYGSATRDVEEGNLNSLD |
B. fuckeliana_a | Â | PGVLPDVDDTSKGLEAL | VLSILNFHADEYMEGIIERH |
X. parietina_a | RRMLSSWDATSSDDVGFEVL | PSVLDESDDTGSSIYIL | VFSALLYDFDHYMEDIIAGF |
Z. passerinii_a | ASQLASWNDVEITNHIGVEL | PACSRTMMDQHANGSWE | YLIRDHSSTMNSRDSA |