Fig. 2

Promoter regions employed in this study. The H. pylori 26695 ureI promoter region is previously characterised, the C. jejuni 11168 porA and H. pullorum NCTC 12824 flaA promoters are putative based on known consensus sequences. Boxed sequences denote known or putative −10 and −35 regions with the start codon of the associated open reading frames in bold capitals. Sequences were amplified by PCR to include 5′ and 3′ restriction sites and italicised bases were altered to “CAT” to create an NdeI site at the 3′ end