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Table 3 Percent recovery and limit of detection for mock community spiked into whole blood

From: The development and application of a molecular community profiling strategy to identify polymicrobial bacterial DNA in the whole blood of septic patients

  Percent recovery (%) Limit of detection (CFU/ml)
Saponin alone Saponin + 1 Wash Saponin + 2 washes Saponin alone Saponin +1 wash Saponin + 2 washes
Organism SC1 SC3 SC5 SC1 SC3 SC5 SC1 SC3 SC5  
E. coli 82.8 100 73.7 24.8 0 0 32.3 51.7 15.5 102 104 103
E. hormaechei 100 100 100 100 100 100 100 100 100 10 10 10
N. flava 100 100 23.4 100 100 75.6 100 100 37.8 105 104 104
M. luteus 100 68.7 8.0 100 6.9 0 20.1 0 0 102 10 10
S. epidermidis 100 100 100 100 100 39.6 100 100 100 10 10 10
S. aureus 100 100 39.3 100 100 31.7 100 100 100 10 10 10
S. intermedius 100 100 100 100 100 19.9 100 100 100 10 10 10
S. pneumoniae 100 100 0 96.9 100 0 100 100 100 102 102 10
F. necrophorum a 0 100 0 0 0 0 0 0 0 102 103 104
P. melaninogenica a 100 100 0 100 0 0 0 0 0 102 102 102
  1. aBased on recovery alone (Fig. 3) not in SC