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Fig. 2 | BMC Microbiology

Fig. 2

From: The development and application of a molecular community profiling strategy to identify polymicrobial bacterial DNA in the whole blood of septic patients

Fig. 2

Enzymatic digestion requirement for bacterial DNA extraction of whole blood. Whole blood was spiked with a CBA plate pool from ASN087 BAL sample. Following a treatment with saponin, the resulting pellet was either digested with no enzyme (black), lysozyme (grey), or lysozyme and mutanolysin (blue). More T-RFs were present when enzymatic digestion was done with lysozyme (10, grey) and lysozyme plus mutanolysin (24, blue) identified, respectively, when compared to no enzymatic treatment with only 7 T-RFs present

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