Fig. 5

Quantitative real time-PCR assays. Gene expressions were determined in the M. smegmatis Ms0535 deletion strain (a), in the Ms0535-overexpressing strain (b), and in wild type strain with or without isoniazid (c). The relative expression levels of the genes were normalized using the sigA gene as an invariant transcript. An unrelated promoter of the Ms0540 gene was used as a negative control. Data were analyzed using the 2^−ΔΔCt method as described in the Methods. P-values of the relative expression data were calculated by an unpaired two-tailed Student’s t-test