Fig. 1

Schematic representation of the carAB operon and the RT-PCR strategy. a The grey rectangles depicted the open reading frames of the operon and their lengths in base pairs. The arrows represent the sizes and approximate locations in the PCR analysis with primer sets. All forward and reverse primers were gene-specific. b PCR products by using gDNA as template. c and d PCR products from first strand cDNA. The DNA marker was DL5000