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Table 2 Fatty acid composition of total lipid extracts from R. solanacearum fabG mutant strains grown on M63 medium plates a

From: Ralstonia solanacearum fatty acid composition is determined by interaction of two 3-ketoacyl-acyl carrier protein reductases encoded on separate replicons

Fatty acid RS-G2 (fabG2::Gm) RS-G3 (fabG1::EcfabG) RS-G5 (fabG1::Gm/pYJ33) GMI1000
n-C14:0 2.32 ± 0.09 3.00 ± 0.10 2.99 ± 0.13 2.38 ± 0.40
n-C14:0 3-OH 32.53 ± 2.24 21.49 ± 0.28 29.68 ± 2.54 39.00 ± 2.87
n-C16:1 cis 9 11.54 ± 1.16 16.88 ± 0.48 20.82 ± 1.50 10.52 ± 1.11
n-C16:0 23.25 ± 1.25 28.71 ± 1.94 22.76 ± 1.75 28.47 ± 3.74
n-C16:0 2-OH 2.14 ± 0.44 1.59 ± 0.09 1.03 ± 0.12 2.06 ± 0.16
n-C17:0 cyclo 5.76 ± 0.73 5.51 ± 0.74 1.14 ± 0.24 1.89 ± 0.17
n-C18:1 cis 11 17.20 ± 2.43 17.37 ± 2.12 16.96 ± 0.67 8.98 ± 0.96
n-C18:0 4.21 ± 0.36 4.62 ± 0.29 4.31 ± 0.29 6.54 ± 0.89
UFA/SFAb 1.16 1.09 1.29 0.57
HFA/FA 0.57 0.35 0.44 0.70
  1. aCells were grown on M63 plates at 30 °C for two days. The total lipids were extracted and transesterified to obtain fatty acid methyl esters, and products were identified by GC-MS. The values are the means ± standard deviations of three independent experiments and the percentages of total fatty acids
  2. bUFA denotes unsaturated fatty acid, including C16:1 cis 9, C17:0 cyclo and C18:1 cis 11. SFA denotes saturated fatty acid, including C14:0, C16:0 and C18:0. HFA denotes hydroxyl-fatty acid, including C14:0 3-OH and C16:0 2-OH