Ralstonia solanacearum fatty acid composition is determined by interaction of two 3-ketoacyl-acyl carrier protein reductases encoded on separate replicons

Table 2 Fatty acid composition of total lipid extracts from R. solanacearum fabG mutant strains grown on M63 medium plates ^a

Fatty acid	RS-G2 (fabG2::Gm)	RS-G3 (fabG1::EcfabG)	RS-G5 (fabG1::Gm/pYJ33)	GMI1000
n-C14:0	2.32 ± 0.09	3.00 ± 0.10	2.99 ± 0.13	2.38 ± 0.40
n-C14:0 3-OH	32.53 ± 2.24	21.49 ± 0.28	29.68 ± 2.54	39.00 ± 2.87
n-C16:1 cis 9	11.54 ± 1.16	16.88 ± 0.48	20.82 ± 1.50	10.52 ± 1.11
n-C16:0	23.25 ± 1.25	28.71 ± 1.94	22.76 ± 1.75	28.47 ± 3.74
n-C16:0 2-OH	2.14 ± 0.44	1.59 ± 0.09	1.03 ± 0.12	2.06 ± 0.16
n-C17:0 cyclo	5.76 ± 0.73	5.51 ± 0.74	1.14 ± 0.24	1.89 ± 0.17
n-C18:1 cis 11	17.20 ± 2.43	17.37 ± 2.12	16.96 ± 0.67	8.98 ± 0.96
n-C18:0	4.21 ± 0.36	4.62 ± 0.29	4.31 ± 0.29	6.54 ± 0.89
UFA/SFA^b	1.16	1.09	1.29	0.57
HFA/FA	0.57	0.35	0.44	0.70

^aCells were grown on M63 plates at 30 °C for two days. The total lipids were extracted and transesterified to obtain fatty acid methyl esters, and products were identified by GC-MS. The values are the means ± standard deviations of three independent experiments and the percentages of total fatty acids
^bUFA denotes unsaturated fatty acid, including C16:1 cis 9, C17:0 cyclo and C18:1 cis 11. SFA denotes saturated fatty acid, including C14:0, C16:0 and C18:0. HFA denotes hydroxyl-fatty acid, including C14:0 3-OH and C16:0 2-OH

ISSN: 1471-2180