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Fig. 3 | BMC Microbiology

Fig. 3

From: Ralstonia solanacearum fatty acid composition is determined by interaction of two 3-ketoacyl-acyl carrier protein reductases encoded on separate replicons

Fig. 3

Purification of R. solanacearum FabGs from E. coli strain BL21 (DE3) and circular dichroism spectra of R. solanacearum FabG1 and FabG2. Panel a, Purification of R. solanacearum GMI1000 FabG1 and FabG2 by native nickel-chelate chromatography. Lane 1, molecular mass markers; lane 2, R. solanacearum FabG1 protein; lane 3, R. solanacearum FabG2 protein. Panel b, Size exclusion chromatography of the hexahistidine-tagged wild type RsFabG1 and FabG2 protein. The elution peaks of the molecular weight standards are given at the top of the panel. Panel c, CD spectra of R. solanacearum FabGs. CD spectra were measured at 25 °C. Open circles, E. coli FabG; filled triangle, R. solanacearum FabG1; open squares, R. solanacearum FabG2

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BMC Microbiology

ISSN: 1471-2180

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