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Table 1 Primers used in PCR detection of Shiga toxin genes and determination of serotypes

From: Multiple antibiotic resistances among Shiga toxin producing Escherichia coli O157 in feces of dairy cattle farms in Eastern Cape of South Africa

Target gene Primer sequences 5′-3′ Size of product (bp) Reference
stx1 STx1-F TTC GCT CTG CAA TAG GTA 555 [18]
STx1-R TTC CCC AGT TCA ATG TAA GAT
stx2 STx2-F GTG CCT GTT ACT GGG TTT TTC TTC 118 [18]
STx2-R AGG GGT CGA TAT CTC TGT CC
rfbE(O157) rfbE-F TTT CAC ACT TAT TGG ATG GTC TCA A 88 [17]
rfbE-R CGA TGA GTT TAT CTG CAA GGT GAT