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Table 1 Primers used in PCR detection of Shiga toxin genes and determination of serotypes

From: Multiple antibiotic resistances among Shiga toxin producing Escherichia coli O157 in feces of dairy cattle farms in Eastern Cape of South Africa

Target gene

Primer sequences 5′-3′

Size of product (bp)

Reference

stx1

STx1-F TTC GCT CTG CAA TAG GTA

555

[18]

STx1-R TTC CCC AGT TCA ATG TAA GAT

stx2

STx2-F GTG CCT GTT ACT GGG TTT TTC TTC

118

[18]

STx2-R AGG GGT CGA TAT CTC TGT CC

rfbE(O157)

rfbE-F TTT CAC ACT TAT TGG ATG GTC TCA A

88

[17]

rfbE-R CGA TGA GTT TAT CTG CAA GGT GAT