Fig. 5

Identification of the yECM proteins changing expression due to the mutation on GUP1 gene. a 2D DIGE analysis of the samples 1 and 2, showing the identification of the protein spots which abundance varied ≥ 2-fold and present p < 0.05. b and c Unsupervised multivariate analysis of data from the 2D DIGE experiment. b Upper panel: Principal Component Analysis showing respectively the clustering of the eight individual Cy3- and Cy5-labeled DIGE spot maps; Lower panel: score plots showing the subset of proteins whose ratios varied 2-fold or more and in which p < 0.05 in the two principle components. c Heat map of the relative protein expression values, each vertical lane corresponding to a sample from A - upper panel, and each horizontal lane a protein spot from A - lower panel. Hierarchical clustering settings are Pearson distance measurements and average linkage. The dendrogram of eight individual spot maps clustering is shown at the top, and that of individual proteins is shown on the left