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Fig. 2 | BMC Microbiology

Fig. 2

From: Quantitative differential proteomics of yeast extracellular matrix: there is more to it than meets the eye

Fig. 2

Analysis of the total yECM proteome from S. cerevisiae wt and gup1∆ mutant. a SDS-PAGE of yECM is compared with cell’s total proteome (CTP) from cells grown in solid media and liquid growth medium (LGM). Each lane displays a different pattern though some bands are clearly common. b 2DE analysis of wt yECM protein extract in lane 2. The protein in marked spots were identified by MALDI-TOF/TOF: Act1 (actin 1), Adh1 (alcohol dehydrogenase 1), Ahp1 (alkyl hiydroperoxide reductase 1), Ald3 (aldehyde dehydrogenase 3), Cit1 (citrate synthase 1), Cpr1 (cyclosporin A-sensitive proline rotamase 1), Eno1 (enolase 1), Eno2 (enolase 2), Hsp26 (heat shock protein 26), Ilv5 (isoleucine-plus-valine requiring enzyme 5), Oye2 (old yellow enzyme 2), Pdc1 (pyruvate decarboxylase 1), Pyk1 (pyruvate kinase 1), Ree1 (regulation of enolase 1), Sod2 (superoxide dismutase 2), Ssa1 stress-seventy subfamily A 1), Tdh3 (triose-phosphate dehydrogenase 3), Tpi1 (triose-phosphate isomerase), and the uncharacterized ORF YNL134C

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