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Fig. 7 | BMC Microbiology

Fig. 7

From: Deciphering the mode of action of a mutant Allium sativum Leaf Agglutinin (mASAL), a potent antifungal protein on Rhizoctonia solani

Fig. 7

mASAL induced PCD in R solani. a Nuclear morphology of R. solani by DAPI staining. First column: R. solani hyphae treated with 20 μg/ml mASAL for different time intervals, 24 h (upper panel), 48 h (middle panel) and 72 h (lower panel). First and third column represent the DAPI stained R. solani hyphae treated with mASAL and PBS (control) respectively. Second and fourth column represent the DIC (differential interference contrast) images of mASAL treated and control sample respectively. Scale bars represent 20 μm. b DNA fragmentation induced by mASAL. Genomic DNA from R. solani cells treated with either 20 μg/ml mASAL for three different time points or PBS for control were run on 1 % agarose gel. Lane 1: DNA of R. solani treated with PBS buffer for 72 h as the control. Lane 2–4: DNA of R. solani treated with mASAL for 24, 48 and 72 h respectively. Lane M represents DNA molecular weight marker. c Annexin-V-FITC assay. The mycelia of R.solani were treated with 20 μg/ml mASAL for 48 h and stained with annexin-V-FITC and Propidium iodide (PI). Upper Left panel: Annexin V-FITC, upper right panel: PI, Lower left panel: DIC (differential interference contrast), Lower right panel: Merge images of FITC/PI and DIC. Bars represent 25 μm

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