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Table 1 Oligonucleotides and plasmids used in this work

From: Serratiopeptidase: a well-known metalloprotease with a new non-proteolytic activity against S. aureus biofilm

Plasmid Description References
pET28b-Spep pET28b (+) containing the Spep gene of S. marcescens E-15 This work
pET28b-Spep-EmutA pET28b (+) containing the Spep-EmutA gene of S. marcescens E-15 This work
pRuW4inh1 Secretion system of Erwinia chrysanthemi [20]
Oligonucleotides   
Spep fw EcoRI/NcoI 5’-GGTAATGAGTGGAATTCAACCCATGGAATCTAC-3’  
Spep rv EcoRI 5’-CATCACCGAATTCGTCACCATCATGC-3’  
Spep_EmutA fw 5’-CGTTTACCCATGCGATTGGCCATGC-3’  
Spep_EmutA rv 5’-GCATGGCCAATCGCATGGGTAAACG-3’  
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