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Table 1 Oligonucleotides and plasmids used in this work

From: Serratiopeptidase: a well-known metalloprotease with a new non-proteolytic activity against S. aureus biofilm

Plasmid

Description

References

pET28b-Spep

pET28b (+) containing the Spep gene of S. marcescens E-15

This work

pET28b-Spep-EmutA

pET28b (+) containing the Spep-EmutA gene of S. marcescens E-15

This work

pRuW4inh1

Secretion system of Erwinia chrysanthemi

[20]

Oligonucleotides

  

Spep fw EcoRI/NcoI

5’-GGTAATGAGTGGAATTCAACCCATGGAATCTAC-3’

 

Spep rv EcoRI

5’-CATCACCGAATTCGTCACCATCATGC-3’

 

Spep_EmutA fw

5’-CGTTTACCCATGCGATTGGCCATGC-3’

 

Spep_EmutA rv

5’-GCATGGCCAATCGCATGGGTAAACG-3’