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Fig. 1 | BMC Microbiology

Fig. 1

From: Serratiopeptidase: a well-known metalloprotease with a new non-proteolytic activity against S. aureus biofilm

Fig. 1

SDS-PAGE and zymogram analyses of supernatants of bacterial cultures harboring pET28b-Spep (lane 1) and pET28b-Spep-EmutA (lane 2), respectively. Panel a SDS-PAGE and Panel b zymogram assays. Mk: precision Plus prestained marker, Biorad (range 250–10 kDa); C: 5 μg of SPEP (2540 U mg−1, Osaka Japan). Panel c Mascot Search Results of the peptide mixtures derived from digestion with trypsin of the 1 and 2 bands performed with LC/MS-MS (3520 Chip Q-Tof, Agilent)

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