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Fig. 4 | BMC Microbiology

Fig. 4

From: Lactobacillus acidophilus attenuates Salmonella-induced intestinal inflammation via TGF-β signaling

Fig. 4

Effects of L. acidophilus and S. typhimurium on SMAD7 expression and SMAD3/4 transcriptional activity. a Human intestinal Caco-2 cells were treated with 1 % inulin or L. acidophilus (L. acidophilus:S. typhimurium = 2:1) or synbiotics (L. acidophilus and 1 % inulin) 1 h prior to treatment with S. typhimurium in DMEM without antibiotic for 1 h. The cells were harvested at 6 h post-infection for protein extraction. SMAD7 protein expression was determined by immunoblotting. b Human intestinal Caco-2 cells were transfected with luciferase reporter plasmid for SMAD3/4 or CMV (normalization control) overnight and then treated or infected as above. The cells were added DMEM medium containing D-luciferin at 6 h post-infection to read the signal with a Luminometer. The quantitative results are shown as the means ± SEM from three independent experiments. NS: not significant

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