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Fig. 1 | BMC Microbiology

Fig. 1

From: Serological diagnosis of pulmonary Mycobacterium tuberculosis infection by LIPS using a multiple antigen mixture

Fig. 1

Schematic of the LIPS assay. DNA sequence of the MTB antigens of interest are genetically fused to the N- or C-terminus of Renilla luciferase (Ruc). These recombinant plasmids are then used to transfect Cos1 cells and cell lysate is harvested without purification. Aliquots of extract containing the Ruc-MTB antigen are then incubated with serum samples. The antibody complexes are then captured by protein A/G beads and the unbound luciferase tagged antigen is removed by extensive washing. The amount of specific antibody is determined by the amount of bound MTB-Ruc antigen present, which is determined by adding luciferase substrate and detection of luminescence

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BMC Microbiology

ISSN: 1471-2180

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