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Fig. 4 | BMC Microbiology

Fig. 4

From: Mutations in the bacterial cell division protein FtsZ highlight the role of GTP binding and longitudinal subunit interactions in assembly and function

Fig. 4

Steady state kinetic analysis of FtsZ, FtsZ84, and FtsZ84* proteins. GTPase assays were carried out as described in materials and methods (50 mM MES, pH 6.5, 50 mM KCl, 2.5 mM MgCl2, 1 mM EGTA). FtsZ concentration was kept constant at 5 μM and GTP concentration were varied from 0.1 μM to 5 μM (different ranges were used depending on the FtsZ mutant analyzed). The best fit for our data was obtained using a Hill-modified Michaelis-Menten equation using Sigma Plot software. Km and Vmax values are listed in Table 3

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