Fig. 5

Verification of expression of gene PP3901 induced by oxidative stress conditions. a Expression of PP3901 in the presence of H₂O₂ (1.5 mM), paraquat (PQ, 100 μM), or without an oxidative stress inducer (control, Ctrl) quantified by RT-qPCR.Data are mean values ± S.D. (n = 3) of mRNA per DNA of the gene in question. Asterics (*) indicate mean values of treated cultures that are significantly (P < 0.05) higher than mean values from Ctrl cultures. b Induction of the bioreporter harboring a transcriptional fusion between PP3901 and msf-gfp in P. putida mt-2 after exposure 1.5 mM H₂O₂, 100 μM PQ (left panel), or 30 μg mL⁻¹ nalidixc acid (right panel). Note the different scales on the y-axis of the diagrams. Data are mean values of average fluorescence intensity from triplicate cultures ± S.D. Asterisks (*) indicate mean values from treated cultures that are significantly (P < 0.05) higher than mean values from Ctrl cultures