Table 2 Primers used in this study
Primer | Sequence (5’-3’)a | Description |
|---|---|---|
attB4_UDPgalFT5F | ggggacaactttgtatagaaaagttgAGAAACTTTAGCCAGAACTT | ugtA 5’ element |
attB1r_UDPgalFT5R | ggggactgcttttttgtacaaacttgAGGACTGACTAGAAGTTCAG | ugtA 5’ element |
attB2r_UDPgalFT3F | ggggacagctttcttgtacaaagtggTGTGAAAGTGCGAGTCTGAT | ugtA 3’ element |
attB3_UDPgalFT3R | ggggacaactttgtataataaagttgGGATTCAGGTCCGGGTCCAG | ugtA 3’ element |
attB4_ugtB5F | ggggacaactttgtatagaaaagttgGGTCGACCAGACTCCACCAA | ugtB 5’ element |
attB1r_ugtB5R | ggggactgcttttttgtacaaacttgGATGGACGGTCGCACACGAG | ugtB 5’ element |
attB2r_ugtB3F | ggggacagctttcttgtacaaagtggAGTCGATTGTACATATGGTA | ugtB 3’ element |
attB3_ugtB3R | ggggacaactttgtataataaagttgGCGACAAGAACACCATTGGC | ugtB 3’ element |
attB4 FW_ugtA5 | ggggacaactttgtatagaaaagttgTGGTCAGTCATTCCCTTTCGAGC | ugtA_CFP_tagging |
attB1 R_ugtA5 | ggggactgcttttttgtacaaacttgAGGCATTTCCAGCAGTAGCGC | ugtA_CFP_tagging |
attB2 FW_ugtA3 | ggggacagctttcttgtacaaagtggAGGACTGACTAGAAGTTCAGG | ugtA_CFP_tagging |
attB3 R_ugtA3 | ggggacaactttgtataataaagttgACCGTACAGTAACAGGTGAC | ugtA_CFP_tagging |
attB4 FW_ugtB5 | ggggacaactttgtatagaaaagttgTGACCTCAGTGTGTCCTTCATCC | ugtB_CFP_tagging |
attB1 R_ugtB5 | ggggactgcttttttgtacaaacttgAGTTCTCGGGGCGGGGGCCAC | ugtB_CFP_tagging |
attB2 FW_ugtB3 | ggggacagctttcttgtacaaagtggAGTCGATTGTACATATGGTA | ugtB_CFP_tagging |
attB3 R _ugtB3 | ggggacaactttgtataataaagttgTACCACCTTCACCCTTGACC | ugtB_CFP_tagging |