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Fig. 3 | BMC Microbiology

Fig. 3

From: Identification and functional analysis of two Golgi-localized UDP-galactofuranose transporters with overlapping functions in Aspergillus niger

Fig. 3

Analysis of Galf-containing glycoconjugates in wildtype and Galf mutants. a Dot blot assay to detect the presence of Galf residues on secreted glycoconjugates from A. niger mutants. A. niger wild-type strain and Galf mutants were grown to early stationary phase and cell-free medium was spotted on nitrocellulose filter paper. The blots were incubated with the anti-Galf antibody (L10) to detect the presence of Galf or incubated with ConA-PO to detect mannoproteins. b Platelia assay with anti-Galf antibody EB-A2 was performed on indicated amounts of purified (galacto)mannan from indicated strains. c The percentage of the monosaccharides (Gal = galactose, Glc = glucose, Man = mannose) detected on HPAEC after hydrolysis of (galacto)mannan from indicated strains is shown. Figures shown are representative for at least two independent experiments

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BMC Microbiology

ISSN: 1471-2180

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