Fig. 1

Gene deletion of ugtA and ugtB in A. niger. a and c Schematic representation of the strategy to disrupt ugtA (a) and ugtB (c) using the hygromycinB selection marker (HygroB) or the pyrG selection marker flanker by repeats of the trpC terminator regions (TtrpC). The 5’ugtA and the 3’ugtB probes used for hybridisation are indicated. Genomic DNA was digested with BamHI (UgtA blot) or XbaI (UgtB blot) and the length of the expected fragments is indicated. b and d Southern blots of genomic DNA after digestion and hybridisation with ³²P-labelled probes. Approximate sizes of the bands based on DNA ladder (not shown) are indicated