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Table 1 Results of PCR and hybridization for EAF-encoded genes among typical EPEC strains

From: Genetic analysis of enteropathogenic Escherichia coli (EPEC) adherence factor (EAF) plasmid reveals a new deletion within the EAF probe sequence among O119 typical EPEC strains

Clonal group Serotype No. of strains Source Presence or absence of: IS element upstream of per Presence or absence of: perBC-EAF PCR product
bfpA (probe) bfpA (PCR) bfpG (PCR) trcP (probe) per (probe) perA (PCR) EAF (PCR) orf35–36 (probe) orf61–62 (probe)
EPEC1 O55:H6, NM 13 Patients + + + IS1(νξ) + + + + + 3.9-kb
EPEC1 O55:NM 1 Control + + + IS1(νξ) + + + + + 3.9-kb
EPEC1 O86:NM, H34 2 Patients + + + + IS1(νξ) + + + + + 3.9-kb
EPEC2 O111:NM 5 Patients + + + + IS1(νξ) + + + + + 3.9-kb
EPEC2 O111:H2 4 Patients + + + + IS1(νξ) + + + + + 3.9-kb
EPEC1 O119:H6, NM 16 Patients + + + IS1(νξ) + + + 2.2-kb
EPEC1 O119:H6 1 Patient + + IS1(νξ) + + 2.2-kb
EPEC1 O119:H6, NM 5 Patients + + + IS1(νξ) + + + 2.2-kb
EPEC1 O119:H6 2 Controls + + + + IS1(νξ) + + + + 3.9-kb
EPEC1 O119:H6 1 Control + + + + IS1(νξ) + + + 3.9-kb
EPEC1 O127:H6, NM 3 Controls + + + IS1(νξ) + + + + 3.9-kb
EPEC1 O127:NM 1 Patient + + + IS1(νξ) + + + + 3.9-kb
Unknown O2:H2, H45 2 Patients + + + IS1(νξ) + + + + 3.9-kb
Unknown O101:H33 1 Control + + + IS1(νξ) + + + + 3.9-kb
Unknown O145:HNT 1 Control + + + IS1(νξ) + + + 2.2-kb
Unknown O162:NM, H33 2 Patients + + + IS1(νξ) + + + + 3.9-kb
Unknown O157:HND 2 Patients + + + IS1(νξ) + + + 2.2-kb
Unknown ONT:H45; HND 3 Patients + + + IS1(νξ) + + + + 3.9-kb
Unknown ONT:HND 5 Patients + + + IS1(νξ) + + + 2.2-kb
EPEC1 E2348/69 (O127:H6) + + + IS1(νξ) + + + + + 3.9-kb
EPEC2 B171 (O111:NM) + + + + IS1(νξ) + + + + + 3.9-kb
  1. NM Nonmotile; ND Nondetermined; NT Nontypeable