Fig. 5From: Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophoroseCellulase detection using indirect immunofluorescence in the Δacy1 mutant strain. The fungus was grown in sophorose for 6 h in the presence or absence of dibutyryl-cAMP (dbcAMP). To detect cellulase in the mycelia we used a rabbit polyclonal anti-Trichoderma viride cellulose (CEL7A) antibody diluted 1:1000, and an Alexa Fluor 488-conjgated secondary antibody diluted 1:5000. Furthermore, the fluorochrome DAPI was used to detect cell nuclei. Images were acquired on a Leica TCS SP5 confocal microscope. Lens 40x, and zoom 3.8Back to article page