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Fig. 1 | BMC Microbiology

Fig. 1

From: Development of a bead-based Luminex assay using lipopolysaccharide specific monoclonal antibodies to detect biological threats from Brucella species

Fig. 1

Antigen-binding properties of the generated Brucella LPS-specific mAbs. a Reactivity of the produced mAbs with extracted B. melitensis or B. abortus LPS in ELISA. b Western blot staining-patterns obtained with mAbs 10G1, 3D12 and 1E2 after SDS-PAGE of extracted B. melitensis and B. abortus LPS. c Indirect immunofluorescent staining of inactivated B. melitensis (16 M) cells by mAbs 3D12 and 10G1. The upper panel shows DNA staining with DAPI, the middle panel Alexa 568-specific immunofluorescence staining and the lower panel merged pictures of both stainings. d Reactivity of mAb 3D12 with gamma, formalin and heat inactivated B. melitensis (16 M) and B. abortus (544) cells in ELISA

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BMC Microbiology

ISSN: 1471-2180

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