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Table 4 Bioinformatic analysis of identified and quantified proteins in the secretome of C. neoformans under Pka1-repressed and Pka1-induced conditions

From: Secretome profiling of Cryptococcus neoformans reveals regulation of a subset of virulence-associated proteins and potential biomarkers by protein kinase A

GO categoriesa Accession number Protein name Signal peptideb (position) GPI anchorc (position) Sample preparation
Carbohydrate catabolic process    
  CNAG_02189 α-Amylase Yes (21/22) Yes (C-26) EtOH, TCA/acetone
GTP catabolic process    
  CNAG_06125 Translation elongation factor 1 α No No EtOH, TCA/acetone
  CNAG_06840 Translation elongation factor 2 No No TCA/acetone
Carbohydrate metabolic process    
  CNAG_00799 Cellulase Yes (22/23) No TCA/acetone
  CNAG_01239 Chitin deacetylase Yes (18/19) Yes (C-28) EtOH, TCA/acetone
  CNAG_02860 Endo-1,3(4)-β-glucanase Yes (22/23) No EtOH
  CNAG_04245 Chitinase Yes (21/22) No EtOH, TCA/acetone
  CNAG_05799 Chitin deacetylase Yes (18/19) Yes (C-28) EtOH
  CNAG_06291 Deacetylase Yes (19/20) No EtOH, TCA/acetone
  CNAG_06313 Phosphoglucomutase No No TCA/acetone
  CNAG_06501 1,3-β-glucanosyltransferase Yes (20/21) Yes (C-6) EtOH, TCA/acetone
Cellular carbohydrate metabolic process    
  CNAG_03225 Malate dehydrogenase No No EtOH, TCA/acetone
Trehalose metabolic process    
  CNAG_03525 Trehalase Yes (18/19) No TCA/acetone
Glyoxylate metabolic process    
  CNAG_01137 Aconitase No No TCA/acetone
Pentose-phosphate pathway    
  CNAG_01984 Transaldolase No No TCA/acetone
  CNAG_07561 Phosphogluconate dehydrogenase No No EtOH
Protein ubiquitination    
  CNAG_01920 Polyubiquitin No No EtOH
ATP hydrolysis coupled proton transport    
  CNAG_05750 ATPase α subunit No No EtOH, TCA/acetone
  CNAG_05918 F0F1 ATP synthase subunit β No No EtOH
Transmembrane transport    
  CNAG_02974 Voltage-dependent ion-selective channel No No TCA/acetone
  CNAG_06101 Eukaryotic ADP/ATP carrier No Yes (C-25) EtOH, TCA/acetone
Methionine biosynthetic process    
  CNAG_01890 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase No No EtOH
Dephosphorylation    
  CNAG_02944 Acid phosphatase Yes (16/17) No EtOH, TCA/acetone
Glycolysis    
  CNAG_03072 Phosphopyruvate hydratase No No EtOH, TCA/acetone
  CNAG_06699 Glyceraldehyde-3-phosphate dehydrogenase No No TCA/acetone
Oxidation-reduction process    
  CNAG_01019 Cu/Zn superoxide dismutase No No EtOH
  CNAG_03465 Laccase Yes (20/21) No EtOH, TCA/acetone
Proteolysis    
  CNAG_00581 Endopeptidase Yes (19/20) No TCA/acetone
  CNAG_00919 Carboxypeptidase D Yes (21/22) No EtOH, TCA/acetone
Response to stress    
  CNAG_00334 Heat shock protein No No TCA/acetone
  CNAG_01727 Hsc70-4 No No EtOH
  CNAG_01750 Chaperone No No EtOH, TCA/acetone
  CNAG_06150 Heat-shock protein 90 No No TCA/acetone
Translation    
  CNAG_04021 60S ribosomal protein L26 No No TCA/acetone
  CNAG_04114 40S ribosomal protein S0 No No TCA/acetone
  CNAG_06095 Ribosomal protein L13 No No EtOH
  CNAG_06605 Ribosomal protein S2 No No TCA/acetone
Regulation of transcription    
  CNAG_00483 Actin No No TCA/acetone
Nucleosome assembly    
  CNAG_06746 Histone h2b No No TCA/acetone
Unknown/Unclassified    
  CNAG_00407 Glyoxal oxidase Yes (16/17) Yes (C-27) EtOH, TCA/acetone
  CNAG_00776 Immunoreactive mannoprotein MP88 Yes (22/23) Yes (C-30) TCA/acetone
  CNAG_01653 Cytokine-inducing glycoprotein Yes (19/20) No EtOH
  CNAG_02030 Glyoxal oxidase Yes (21/22) Yes (C-32) EtOH, TCA/acetone
  CNAG_02850 Glucan endo-1,3-α-glucosidase agn1 Yes (21/22) Yes (C-26) TCA/acetone
  CNAG_02864 Predicted protein Yes (16/17) No TCA/acetone
  CNAG_02943 Cytoplasmic protein No No TCA/acetone
  CNAG_04291 Glycosyl-hydrolase Yes (17/18) Yes (C-31) EtOH
  CNAG_04753 Lactonohydrolase No No EtOH, TCA/acetone
  CNAG_06267 Rds1 protein Yes (20/21) Yes EtOH, TCA/acetone
Hypothetical    
  CNAG_00586 Conserved hypothetical protein Yes (23/24) No EtOH, TCA/acetone
  CNAG_00587 Conserved hypothetical protein Yes (19/20) No TCA/acetone
  CNAG_00588 Conserved hypothetical protein Yes (16/17) No EtOH
  CNAG_01047 Conserved hypothetical protein Yes (19/20) No EtOH, TCA/acetone
  CNAG_01562 Conserved hypothetical protein Yes (18/19) No EtOH
  CNAG_03007 Conserved hypothetical protein No No EtOH
  CNAG_03223 Conserved hypothetical protein Yes (19/20) Yes (C-18) EtOH
  CNAG_03492 Conserved hypothetical protein Yes (19/20) No TCA/acetone
  CNAG_05312 Conserved hypothetical protein Yes (19/20) Yes (C-19) EtOH, TCA/acetone
  CNAG_05595 Conserved hypothetical protein Yes (18/19) Yes (C-29) TCA/acetone
  CNAG_05893 Conserved hypothetical protein Yes (16/17) Yes (C-13) EtOH, TCA/acetone
  CNAG_06109 Conserved hypothetical protein No No EtOH
  1. aProteins were categorized based on GO terms associated with their biological classification
  2. bThe presence of a signal peptide was determined using SignalP, Phobius, and Signal-3 L
  3. cThe presence of a signal peptide was determined using GPI-SOM