Skip to main content
Fig. 6 | BMC Microbiology

Fig. 6

From: Secretome profiling of Cryptococcus neoformans reveals regulation of a subset of virulence-associated proteins and potential biomarkers by protein kinase A

Fig. 6

Interactions of WT and Pka1-regulated strains with J774A.1 murine macrophages. a Intracellular rate at 2 hpi of WT and P GAL7 ::PKA1 strains grown under Pka1-repression (glucose) and Pka1-induction (galactose). b As a control, the colony forming units (CFUs) per macrophage grown in standard DMEM medium (containing 0.45 % glucose) are presented. c Rate of intracellular fungal cell per macrophage at 24 hpi of WT and P GAL7 ::PKA1 strains grown under Pka1-repression (glucose) and Pka1-induction (galactose). d The CFUs per macrophage grown in standard DMEM medium (containing 0.45 % glucose) are presented as a control. The experiments were performed in triplicate; the average percent of survival was reported ± standard error of the mean. For statistical analysis, an unpaired t-test with Welch’s correction (p-value < 0.05) was performed between conditions (* denotes significant difference). The samples at 24 hpi were employed for the analysis of protein abundance shown in Fig. 7

Back to article page