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Fig. 3 | BMC Microbiology

Fig. 3

From: Fibronectin-, vitronectin- and laminin-binding proteins at the cell walls of Candida parapsilosis and Candida tropicalis pathogenic yeasts

Fig. 3

Interactions of Candida spp. cell wall-associated proteins with fibronectin (FN), vitronectin (VTN) and laminin (LAM). In panels a and b, the plots are presented for saturable binding of biotinylated cell wall-associated proteins extracted from filamentous forms of C. albicans, C. parapsilosis and C. tropicalis to immobilized FN (a) and of cell wall-associated proteins of C. parapsilosis and C. tropicalis to immobilized VTN and LAM (b) (5 pmoles of FN, VTN or LAM adsorbed into wells of MaxiSorp microplates with the unoccupied surfaces blocked with BSA). Panels c and d show the respective plots for the displacement of biotinylated cell wall-associated proteins (40 μg/ml for FN binding or 15 μg/ml for VTN and LAM binding) from microplate-immobilized ECMPs (3 pmoles of FN or 1.25 pmoles of VTN or LAM per well) by soluble FN, VTN and LAM added at increasing concentrations. Wells without immobilized ECMPs but coated with BSA served as controls and the values obtained for those wells were subtracted from the total binding values. Results from representative experiments are presented, in which data points represent mean values from three determinations (three wells) ± standard deviation

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