Fig. 2
Involvement of the major C. parapsilosis and C. tropicalis cell wall components in the binding of ECMPs. Binding of biotinylated fibronectin (FN) (a), vitronectin (VTN) (b) and laminin (LAM) (c) was determined for C. parapsilosis and C. tropicalis pseudohyphae (obtained from 5 × 108 yeast cells) that were pretreated with enzymes that digest the major constituents of the fungal cell wall, namely: (1) a mixture of α1-2,3 mannosidase and α1-6 mannosidase; (2) proteinase K; and (3) β-1,3-glucanase. The cell suspensions were then incubated with 100 nM of each labeled ECMP for 1.5 h at 37 °C. The amount of bound protein was determined using the SA-HRP/TMB detection system. The binding level of the control (untreated) cells was considered 100 %. Bars represent the mean values of three determinations (three independent yeast cultures) ± standard deviation. Statistical significance levels against the control are indicated with *p < 0.05, ***p < 0.001 or ns “not significant”, whereas statistically significant comparisons between two tested species are marked with # (p < 0.05)