Fig. 1From: Role of the major antigenic membrane protein in phytoplasma transmission by two insect vector speciesImmunofluorescence of A416 antibody and chrysanthemum yellows phytoplasma (CYP) in vibratome sections of Macrosteles quadripunctulatus midguts. In a and b, labeling of CYP cells after 4 h insect feeding on CYP-infected plants. In c and d, A416 antibody acquired through 24 h artificial feeding was directly labeled with a goat anti rabbit FITC-conjugated antibody. In e-h, direct immunofluorescence labeling of A416 antibody with a goat anti rabbit Alexa 633-conjugated antibody (h) followed by CYP labeling (FITC, g) shows the coincidence of the respective signals (f) in insects pre-fed on A416 antibody (24 h) and then on CYP-infected plant (4 h). i, l and m, n are negative controls in which primary and secondary antibody (respectively) was omitted. a, c, e, i, m show bright-field images; b, d, g, h, l, n show fluorescence images; f is the overlay of light and fluorescence images. Bars represent 20 μmBack to article page