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Table 3 Efficacy of the lacI q-IPTG system to modulate pleD* expression

From: Mini-Tn7 vectors for stable expression of diguanylate cyclase PleD* in Gram-negative bacteria

Strain

Relative pleD* expression1

Intracellular c-di-GMP 2

EPS production3

IPTG -

IPTG +

IPTG -

IPTG +

IPTG -

IPTG +

Sme Tn7pleD*Km pBBR1MCS5

15,11 ± 1,98

n.d.

317,16 ± 13,29

n.d.

2,66 × 106 ± 6,9 × 104

2,64 × 106 ± 7,34 × 104

Sme Tn7pleD*Km pBBRlacIq

1,00 ± 0,26

6,08 ± 1,06

55,10 ± 16,27

152,05 ± 8,50

3,51 × 105 ± 1,19 × 104

2,23 × 106 ± 6,36 × 104

Sme Tn7Km

-

-

-

n.d.

6,20 × 104 ± 5,54 × 103

7,39 × 104 ± 3,44 × 103

  1. 1Relative expression (fold change) to Sme Tn7pleD*Km pBBRlacIq strain without IPTG (repression state) by qRT-PCR. In all strains pleD* expression was normalized with to 16S rRNA levels
  2. 2pmol of c-di-GMP/mg of total protein. The c-di-GMP levels of Sme Tn7Km is under the technical limit of detection
  3. 3CF-derived Fluorescence (arbitrary units)
  4. n.d., not determined