Table 1 Transposition efficiencies of mini-Tn7 constructs
From: Mini-Tn7 vectors for stable expression of diguanylate cyclase PleD* in Gram-negative bacteria
Recipient strain | Mini-Tn7 | Frequencya |
|---|---|---|
R. etli CFN42 (Ret) | Tn7pleD* | 3,0 x 10−4 |
Tn7pleD*Km | 6,5 x 10−6 | |
Tn7Km | 5,2 x 10−6 | |
Tn7pleD*Tc | 9,8 x 10−8 | |
Tn7Tc | 5,1 x 10−6 | |
R. leguminosarum bv. viciae UPM791 (Rle) | Tn7pleD*Km | 2,5 x 10−8 |
Tn7Km | 4,8 x 10−8 | |
Tn7pleD*Tc | <10−9 | |
Tn7Tc | 1,8 x 10−9 | |
S. meliloti 8530 (Sme) | Tn7pleD*Km | 4 x 10−8 |
Tn7Km | 8 x 10−8 | |
Tn7pleD*Tc | 3,5 x 10−9 | |
Tn7Tc | 2,3 x 10−8 | |
P. syringae pv. tomato DC3000 (Pto) | Tn7pleD*Km | 1,6 x 10−7 |
Tn7Km | 4,8 x 10−5 | |
Tn7pleD*Tc | 7 x 10−8 | |
Tn7Tc | 2 x 10−5 |