Fig. 2
From: Mini-Tn7 vectors for stable expression of diguanylate cyclase PleD* in Gram-negative bacteria
Quantification of intracellular c-di-GMP levels. c-di-GMP contents of Rhizobium etli CFN42 (Ret), Sinorhizobium meliloti 8530 (Sme), Rhizobium leguminosarum bv. viciae UPM791 (Rle) and Pseudomonas syringae pv. tomato DC3000 (Pto), with plasmid-encoded (pJBpleD*) or chromosomally integrated pleD* gene. Tc transposants in rhizobial strains displayed comparable phenotypes to Km and their c-di-GMP levels were not determined. Control strains without pleD* carried mini-Tn7Km or mini-Tn7Tc. Standard error of three biological replicates are shown