Fig. 1
From: Mini-Tn7 vectors for stable expression of diguanylate cyclase PleD* in Gram-negative bacteria
Mini-Tn7 delivery constructions originated from pUC18T-mini-Tn7T suicide plasmid. The pleD* gene, under the lac promoter, was cloned between the Tn7 Left and Right ends. Downstream pleD* Tetracycline and Kanamycin resistance genes were cloned to facilitate selection. Vectors with resistance genes but without pleD*, were obtained after removing a NcoI internal fragment to pleD*. ApR, KmR, TcR stand for resistance to Ampicillin, Kanamycin and Tetracycline, respectively; MCS, multi-cloning site; T0T1, transcriptional terminators from bacteriophage λ and E. coli rrnB operon, respectively; Tn7L and Tn7R, left and right ends of Tn7 transposon, respectively; P, lac promoter